Combined single cell and single particle ICP-TQ-MS analysis to quantitatively evaluate the uptake and biotransformation of tellurium nanoparticles in bacteria

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dc.contributor.authorGómez Gómez, Beatriz
dc.contributor.authorCorte-Rodríguez, Mario
dc.contributor.authorPérez Corona, María Teresa
dc.contributor.authorBettmer, Jörg
dc.contributor.authorMontes-Bayón, María
dc.contributor.authorMadrid Albarrán, María Yolanda
dc.date.accessioned2024-01-15T11:09:10Z
dc.date.available2024-01-15T11:09:10Z
dc.date.issued2020-07-10
dc.description.abstractAssessing the impact of nanoparticles in living systems implies a proper evaluation of their behaviour at single-cell level. Due to the small size of nanoparticles, their accumulation, transformation and location within single cells is challenging. In this work, the combination of single cell/single particle triple quadrupole inductively coupled plasma mass spectrometry (SC/SP-ICP-TQ-MS) analysis along with X-ray diffraction (XRD) and transmission electron microscopy (TEM) measurements has been applied to go deeper into the uptake and biotransformation of tellurium nanoparticles (TeNPs) in two bacterial model organisms, S. aureus and E. coli. The use of SC-ICP-TQ-MS enabled the individual introduction of bacterial cells where tellurium and phosphorous (as constitutive element) were monitored and detected at concentration levels down to femtogram (fg) per cell. S.aureus uptake of TeNPs was 0.5–1.9 fg Te cell−1 and 7–30 fg Te cell−1 in presence of 0.5 and 15 mg Te L−1 of TeNPs, respectively, whereas for E. coli, the amount of Te ranged from 0.08 to 0.88 fg Te cell−1 and from 2 to 36 fg Te cell−1 in presence of 0.5 and 15 mg Te L−1 of TeNPs, respectively. TEM and XRD analysis confirmed the occurrence of TeNPs biotransformation (from nanospheres to nanorods) as the nanoparticles were incorporated into both bacterial strains. Finally, SP-ICP-MS analysis after cell lysis was applied to determine the number of particles/rods per bacteria cell and to perform the dimensional characterization of the rod-shaped TeNPs. The results obtained clearly confirmed high cell-to-cell variability in terms of Te nanorods dimensions and TeNPs uptake. To the best of our knowledge, this is the first time that SC/SP-ICP-TQ-MS along with TEM and XRD analysis have been applied to investigate, quantitatively, nanoparticle uptake in bacterial cells and to estimate the dimensions of biogenic Te nanorods.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.sponsorshipSpanish Ministry for Science, Innovation and Universities
dc.description.sponsorshipCommunity of Madrid
dc.description.sponsorshipAsturian Foundation for Biosanitary Research and Innovation (FINBA)
dc.description.statuspub
dc.identifier.citationGomez-Gomez, B., Corte-Rodríguez, M., Perez-Corona, M. T., Bettmer, J., Montes-Bayón, M., & Madrid, Y. (2020). Combined single cell and single particle ICP-TQ-MS analysis to quantitatively evaluate the uptake and biotransformation of tellurium nanoparticles in bacteria. Analytica Chimica Acta, 1128, 116-128.
dc.identifier.doi10.1016/j.aca.2020.06.058
dc.identifier.issn0003-2670
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93027
dc.issue.number2020
dc.journal.titleAnalytica Chimica Acta
dc.language.isoeng
dc.page.final128
dc.page.initial116
dc.publisherElsevier
dc.relation.projectID(CTQ2017-83569-C2-1-R, RTI2018-094605-B-I00, FC-GRUPIN-IDI/ 2018/000242)
dc.relation.projectIDEuropean funding from FSE and FEDER programs (project S2018/BAA-4393,AVANSECAL-IICM)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordSingle cell ICP-MS
dc.subject.keywordSingle particle ICP-MS
dc.subject.keywordTellurium nanoparticles
dc.subject.keywordNanorods
dc.subject.keywordS.aureus
dc.subject.keywordE.coli
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleCombined single cell and single particle ICP-TQ-MS analysis to quantitatively evaluate the uptake and biotransformation of tellurium nanoparticles in bacteria
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1128
dspace.entity.typePublication
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relation.isAuthorOfPublication4a062aa2-e9bf-40b4-b63b-6be2c616d57c
relation.isAuthorOfPublication190287de-ed47-4954-86ff-d4f1eddff035
relation.isAuthorOfPublication.latestForDiscovery9a18fb23-8d8f-44c8-b11f-10ac2e8320c7
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