Arginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen

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dc.contributor.authorBobolea, Irina
dc.contributor.authorBarranco, Pilar
dc.contributor.authorPastor Vargas, Carlos
dc.contributor.authorIraola, Victor
dc.contributor.authorVivanco Martínez, Fernando
dc.contributor.authorQuirce, Santiago
dc.date.accessioned2024-07-23T09:56:50Z
dc.date.available2024-07-23T09:56:50Z
dc.date.issued2010-12-23
dc.description.abstractBackground: We report a 31-year-old farmer whose work consists in handling cereal and vegetables, who consulted our clinic because of asthma symptoms after inhalation of dust during manipulation of the deposited material, usually inside the warehouse. Methods and results: Skin prick tests and specific immunoglobulin E (IgE) determinations were negative with common aeroallergens. The patient noted the presence of many spiders in the warehouse, which were identified as the cellar spider Holocnemus pluchei and the common house spider, Tegenaria domestica. Extracts of spider bodies brought in by the patient were obtained and used to perform in vivo and in vitro studies. Molecular characterization of IgE-binding bands was performed by mass spectrometry. We obtained positive prick tests to the extracts of the bodies of both spiders. Immunoblotting displayed different bands in both spider extracts, in a range of 20-70 kDa. All were hemocyanins, except for a 17-kDa protein of Holocnemus identified as an arginine kinase (AK). Bronchial challenge was positive with the extract of the cellar spider and with the AK, but was negative with the domestic house spider. Conclusion: We present the first case of respiratory allergy due to sensitization to AK from a common spider, confirmed by bronchial provocation tests.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.fundingtypePagado por el autor
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationBobolea I, Barranco P, Pastor-Vargas C, Iraola V, Vivanco F, Quirce S. Arginine kinase from the cellar spider (Holocnemus pluchei): a new asthma-causing allergen. Int Arch Allergy Immunol. 2011;155(2):180-6. doi: 10.1159/000319822. Epub 2010 Dec 23. PMID: 21196763.
dc.identifier.doi10.1159/000319822
dc.identifier.issn1018-2438
dc.identifier.issn1423-0097
dc.identifier.officialurlhttps://karger.com/iaa/article/155/2/180/165983/Arginine-Kinase-from-the-Cellar-Spider-Holocnemus
dc.identifier.urihttps://hdl.handle.net/20.500.14352/107027
dc.issue.number2
dc.journal.titleInt Arch Allergy Immunol
dc.language.isoeng
dc.page.final186
dc.page.initial180
dc.publisherKarger
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu612.017
dc.subject.cdu616-056.4
dc.subject.keywordArginine kinase
dc.subject.keywordAsthma
dc.subject.keywordCellar spider
dc.subject.keywordHolocnemus pluchei
dc.subject.keywordOccupational asthma
dc.subject.keywordSpider allergy
dc.subject.ucmAlergología
dc.subject.unesco3207.01 Alergias
dc.titleArginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number155
dspace.entity.typePublication
relation.isAuthorOfPublication25af78c7-0077-4891-a14e-bcd8e51fe408
relation.isAuthorOfPublication6f3e7679-cbc7-4f23-8355-2de0876d46ad
relation.isAuthorOfPublication.latestForDiscovery25af78c7-0077-4891-a14e-bcd8e51fe408
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