Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein
dc.contributor.author | Peltomaa, Riikka Johanna | |
dc.contributor.author | Fikacek, Sabrina | |
dc.contributor.author | Benito Peña, María Elena | |
dc.contributor.author | Barderas, Rodrigo | |
dc.contributor.author | Head, Trajen | |
dc.contributor.author | Deo, Sapna | |
dc.contributor.author | Daunert, Sylvia | |
dc.contributor.author | Moreno Bondi, María Cruz | |
dc.date.accessioned | 2024-01-12T11:26:58Z | |
dc.date.available | 2024-01-12T11:26:58Z | |
dc.date.issued | 2020 | |
dc.description.abstract | The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng/mL (corresponding to 420 μg/kg in food samples) and an IC50 value of 11.0 ng/mL. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained. | |
dc.description.department | Depto. de Química Analítica | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | FALSE | |
dc.description.sponsorship | Universidad Complutense de Madrid | |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.description.sponsorship | Foundation for the National Institutes of Health (USA) | |
dc.description.status | pub | |
dc.identifier.doi | 10.1007/s00604-020-04538-7 | |
dc.identifier.essn | 1436-5073 | |
dc.identifier.issn | 0026-3672 | |
dc.identifier.officialurl | https://doi.org/10.1007/s00604-020-04538-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/92751 | |
dc.issue.number | 547 | |
dc.language.iso | eng | |
dc.page.final | 11 | |
dc.page.initial | 1 | |
dc.publisher | Springer | |
dc.relation.projectID | RTI2018–096410-B-C21 | |
dc.relation.projectID | National Institutes of Health, NIGMS R01GM114321 | |
dc.relation.projectID | National Institutes of Health, NIGMS R01GM127706 | |
dc.relation.projectID | National Science Foundation grant (CHE-1506740, CBET-1841419) | |
dc.rights.accessRights | open access | |
dc.subject.cdu | 543 | |
dc.subject.keyword | Bioluminescence | |
dc.subject.keyword | Gaussia luciferase | |
dc.subject.keyword | Mycotoxin | |
dc.subject.keyword | Zearalenone | |
dc.subject.keyword | Food safety | |
dc.subject.ucm | Química analítica (Química) | |
dc.subject.unesco | 2301 Química Analítica | |
dc.subject.unesco | 33 Ciencias Tecnológicas | |
dc.subject.unesco | 33 Ciencias Tecnológicas | |
dc.subject.unesco | 32 Ciencias Médicas | |
dc.subject.unesco | 31 Ciencias Agrarias | |
dc.title | Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein | |
dc.type | journal article | |
dc.type.hasVersion | AM | |
dc.volume.number | 187 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 4f6d77c5-1abc-4cab-9a5c-eacb835fce30 | |
relation.isAuthorOfPublication | ebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85 | |
relation.isAuthorOfPublication | 8766057b-6628-4a02-a6db-20bddfaf3054 | |
relation.isAuthorOfPublication.latestForDiscovery | 8766057b-6628-4a02-a6db-20bddfaf3054 |
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