Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein

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dc.contributor.authorPeltomaa, Riikka Johanna
dc.contributor.authorFikacek, Sabrina
dc.contributor.authorBenito Peña, María Elena
dc.contributor.authorBarderas, Rodrigo
dc.contributor.authorHead, Trajen
dc.contributor.authorDeo, Sapna
dc.contributor.authorDaunert, Sylvia
dc.contributor.authorMoreno Bondi, María Cruz
dc.date.accessioned2024-01-12T11:26:58Z
dc.date.available2024-01-12T11:26:58Z
dc.date.issued2020
dc.description.abstractThe development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng/mL (corresponding to 420 μg/kg in food samples) and an IC50 value of 11.0 ng/mL. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedFALSE
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.sponsorshipFoundation for the National Institutes of Health (USA)
dc.description.statuspub
dc.identifier.doi10.1007/s00604-020-04538-7
dc.identifier.essn1436-5073
dc.identifier.issn0026-3672
dc.identifier.officialurlhttps://doi.org/10.1007/s00604-020-04538-7
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92751
dc.issue.number547
dc.language.isoeng
dc.page.final11
dc.page.initial1
dc.publisherSpringer
dc.relation.projectIDRTI2018–096410-B-C21
dc.relation.projectIDNational Institutes of Health, NIGMS R01GM114321
dc.relation.projectIDNational Institutes of Health, NIGMS R01GM127706
dc.relation.projectIDNational Science Foundation grant (CHE-1506740, CBET-1841419)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordBioluminescence
dc.subject.keywordGaussia luciferase
dc.subject.keywordMycotoxin
dc.subject.keywordZearalenone
dc.subject.keywordFood safety
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.subject.unesco33 Ciencias Tecnológicas
dc.subject.unesco33 Ciencias Tecnológicas
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco31 Ciencias Agrarias
dc.titleBioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number187
dspace.entity.typePublication
relation.isAuthorOfPublication4f6d77c5-1abc-4cab-9a5c-eacb835fce30
relation.isAuthorOfPublicationebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85
relation.isAuthorOfPublication8766057b-6628-4a02-a6db-20bddfaf3054
relation.isAuthorOfPublication.latestForDiscovery8766057b-6628-4a02-a6db-20bddfaf3054
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