Diadenosine polyphosphates in the tears of aniridia patients

Abstract

Purpose: To quantify diadenosine polyphosphate levels in tears of congenital aniridia patients to estimate the ocular surface changes associated with congenital aniridia compared to normal individuals.

Methods: Fifteen patients diagnosed with congenital aniridia and a control group of forty volunteers were studied. Tears were collected to quantify the levels of diadenosine polyphosphates Ap4A and Ap5A by high-performance liquid chromatography (H.P.L.C). Break-up time (BUT), corneal staining, McMon- nies questionnaire and the Schirmer I test were applied to both groups. Results: Dinucleotides in congenital aniridia patients were higher than in control subjects. For the congenital aniridia group, under 15 years old, the values were 0.77 0.01 lM and 0.17 0.02 lM for Ap4A and Ap5A, respectively. The group aged from 15 to 40 years old provided concentrations of 4.37 0.97 lM and 0.46 0.05 lM for Ap4A and Ap5A, the group over 40 gave concentrations of 11.17 5.53 lM and 0.68 0.17 lM for Ap4A and Ap5A. Dinucleotide concentrations increased with age, being statistically significant different among the three age groups (p < 0.05). Congenital aniridia patients showed a normal tear secretion and no dry eye McMonnies scores, except for the group over 40 years old. BUT values decreased and corneal staining increased with age and correlated with the levels of diadenosine polyphosphates (p < 0.05).

Conclusions: The levels of dinucleotides in tears increase in aniridia patients compared with healthy subjects, and they seem to be related with the progression of corneal disorders in aniridia patients, both of which increase with ageing.