Selection of Recombinant Antibodies by Phage Display Technology and Application for Detection of Allergenic Brazil Nut (Bertholletia excelsa) in Processed Foods

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dc.contributor.authorCruz, Silvia de la
dc.contributor.authorLópez-Calleja, Inés María
dc.contributor.authorAlcocer, Marcos
dc.contributor.authorGonzález Alonso, María Isabel
dc.contributor.authorMartín De Santos, María Del Rosario
dc.contributor.authorGarcía Lacarra, Teresa
dc.date.accessioned2024-01-19T19:28:34Z
dc.date.available2024-01-19T19:28:34Z
dc.date.issued2013
dc.description.abstractCurrent immunological methods for detection of Brazil nut allergens in foods are based on polyclonal antibodies raised in animals. Phage display technology allows the procurement of high-affinity antibodies avoiding animal immunization steps and therefore attaining the principle of replacement supported by animal welfare guidelines. In this study, we screened Tomlinson I and J libraries for specific binders against Brazil nut by employing a Brazil nut protein extract and a purified Brazil nut 2S globulin, and we successfully isolated a phage single chain variable fragment (named BE95) that specifically recognizes Brazil nut proteins. The selected phage scFv was further used as affinity probe to develop an indirect phage-ELISA for detection of Brazil nut in experimental binary mixtures and in commercial food products, with a limit of detection of 5 mg g(-1). This study describes for the first time the isolation of recombinant antibody fragments specific for an allergenic tree nut protein from a naïve library and paves the way to develop new immunoassays for food analysis based on probes that can be produced in vitro when required and do not rely on animal immunization.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationde la Cruz, S., López-Calleja, I. M., Alcocer, M., González, I., Martín, R., & García, T. (2013). Selection of recombinant antibodies by phage display technology and application for detection of allergenic Brazil nut (Bertholletia excelsa) in processed foods. Journal of agricultural and food chemistry, 61(43), 10310–10319. https://doi.org/10.1021/jf403347t
dc.identifier.doi10.1021/jf403347t
dc.identifier.essn1520-5118
dc.identifier.issn0021-8561
dc.identifier.officialurlhttps://www.doi.org/10.1021/jf403347t
dc.identifier.pmid24090075
dc.identifier.relatedurlhttps://pubs.acs.org/doi/10.1021/jf403347t
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94172
dc.issue.number43
dc.journal.titleJournal of Agricultural and Food Chemistry
dc.language.isoeng
dc.page.final10319
dc.page.initial10310
dc.publisherACS Publications
dc.relation.projectIDAGL 2010-15279
dc.relation.projectID2009/AGR-1489
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordBrazil nut
dc.subject.keywordBertollethia excelsa
dc.subject.keywordPhage display
dc.subject.keywordscFv
dc.subject.keywordELISA
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco31 Ciencias Agrarias
dc.titleSelection of Recombinant Antibodies by Phage Display Technology and Application for Detection of Allergenic Brazil Nut (Bertholletia excelsa) in Processed Foods
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number61
dspace.entity.typePublication
relation.isAuthorOfPublicationad71b5da-a51a-461c-a765-dad9886d3d7a
relation.isAuthorOfPublication6f333fdc-ceff-44c3-bf40-41df75f7eb33
relation.isAuthorOfPublication901e6544-8ac8-4029-bf26-1d9d096fe78e
relation.isAuthorOfPublication.latestForDiscoveryad71b5da-a51a-461c-a765-dad9886d3d7a
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