In vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems

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dc.contributor.authorAlonso Gómez, Ángel Luis
dc.contributor.authorCastex, C. Baca
dc.contributor.authorFerrante, A.
dc.contributor.authorPinto, M.
dc.contributor.authorCastañeira, C.
dc.contributor.authorTrasorras, V.
dc.contributor.authorGambarotta, M. C.
dc.contributor.authorLosinno, L.
dc.contributor.authorMiragaya, M.
dc.date.accessioned2023-06-19T15:13:21Z
dc.date.available2023-06-19T15:13:21Z
dc.date.issued2015
dc.description.abstractThe aim of this work was to evaluate the use of air-dried spermatozoa for in vitro production of equine embryos and verify if sperm extract activation and in vivo culture improve in vitro embryo production. Cooled spermatozoa (con-trol) and air-dried spermatozoa stored for 2, 14 or 28 days were used for ICSI sperm extract, or ionomycin was used for oocyte activation, and embryos were in vitro or in vivo (in mare0s oviduct) cultured for 7 days. With in vitro cul-ture, cleavage rate was higher when activating with sperm extract (P < 0.05). No differences in embryo development were seen between the two activation treatments nor between storage periods (P > 0.05). Blastocysts were obtained with cooled spermatozoa, and morulae were achieved using in vivo culture with 28-day storage spermatozoa and ionomycin-activated oocytes. When in vivo culture was performed, sperm DNA fragmentation was assessed using the sperm chromatin dispersion test and did not show statistical correlation with cleavage nor embryo recovery rates. In conclusion, equine embryos can be pro-duced using air-dried spermatozoa stored for several weeks. Sperm extract acti-vation increased cleavage rates but did not improve embryo development. In vivo culture allowed intrauterine stage embryos to be achieved.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipUniversity of Buenos Aires
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/46370
dc.identifier.doi10.1111/and.12273
dc.identifier.issn0303-4569
dc.identifier.officialurlhttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0272
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35567
dc.journal.titleAndrologia
dc.language.isoeng
dc.page.final394
dc.page.initial387
dc.publisherWiley Online Library
dc.relation.projectIDUBACyT V049, VO21
dc.rights.accessRightsrestricted access
dc.subject.cdu591.1
dc.subject.keywordAir-dried spermatozoa
dc.subject.keywordequine
dc.subject.keywordICSI
dc.subject.keywordin vivo culture
dc.subject.keywordsperm extract
dc.subject.ucmFisiología animal (Biología)
dc.subject.unesco2401.13 Fisiología Animal
dc.titleIn vitro equine embryo production using air-dried spermatozoa, with different activation protocols and culture systems
dc.typejournal article
dc.volume.number47
dspace.entity.typePublication
relation.isAuthorOfPublication71c5047d-4eb1-48ac-86d4-21c7b2d335a7
relation.isAuthorOfPublication.latestForDiscovery71c5047d-4eb1-48ac-86d4-21c7b2d335a7
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