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Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo

dc.contributor.authorMuñoz-Sáez, Emma
dc.contributor.authorMoracho, Natalia
dc.contributor.authorLearte, Ana
dc.contributor.authorArroyo, Alicia
dc.contributor.authorSánchez-Camacho Blázquez, Cristina
dc.date.accessioned2024-02-05T12:27:21Z
dc.date.available2024-02-05T12:27:21Z
dc.date.issued2021
dc.descriptionThis research was funded by grants from the UEM (2018UEM13, 2019UEM10) to C.S.-C. and funding from MICINN grants SAF2017-83229-R and PID2020-112981RB-I00 to A.G.A.
dc.description.abstractMT1-MMP/MMP14 belongs to a subgroup of the matrix metalloproteinases family that presents a transmembrane domain, with a cytosolic tail and the catalytic site exposed to the extracellular space. Deficient mice for this enzyme result in early postnatal death and display severe defects in skeletal, muscle and lung development. By using a transgenic line expressing the LacZ reporter under the control of the endogenous Mt1-mmp promoter, we reported a dynamic spatiotemporal expression pattern for Mt1-mmp from early embryonic to perinatal stages during cardiovascular development and brain formation. Thus, Mt1-mmp shows expression in the endocardium of the heart and the truncus arteriosus by E8.5, and is also strongly detected during vascular system development as well as in endothelial cells. In the brain, LacZ reporter expression was detected in the olfactory bulb, the rostral cerebral cortex and the caudal mesencephalic tectum. LacZ-positive cells were observed in neural progenitors of the spinal cord, neural crest cells and the intersomitic region. In the limb, Mt1-mmp expression was restricted to blood vessels, cartilage primordium and muscles. Detection of the enzyme was confirmed by Western blot and immunohistochemical analysis. We suggest novel functions for this metalloproteinase in angiogenesis, endocardial formation and vascularization during organogenesis. Moreover, Mt1-mmp expression revealed that the enzyme may contribute to heart, muscle and brain throughout development.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipUniversidad Europea de Madrid
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.statuspub
dc.identifier.citationMuñoz-Sáez, E.; Moracho, N.; Learte, A.I.R.; Arroyo, A.G.; Sánchez-Camacho, C. Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo. Cells 2021, 10, 2448. https://doi.org/10.3390/cells10092448
dc.identifier.doi10.3390/cells10092448
dc.identifier.issn2073-4409
dc.identifier.officialurlhttps://doi.org/10.3390/cells10092448
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98922
dc.issue.number2448
dc.journal.titleCells
dc.language.isoeng
dc.publisherMDPI
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu577.112
dc.subject.cdu616.831-006.484
dc.subject.keywordMMP14
dc.subject.keywordMt1-mmp
dc.subject.keywordAngiogenesis
dc.subject.keywordBrain
dc.subject.keywordCardiovascular development
dc.subject.keywordDorsal aorta
dc.subject.keywordEmbryo development
dc.subject.keywordExpression pattern
dc.subject.keywordLimb
dc.subject.keywordMetalloproteinases
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmOncología
dc.subject.unesco2403 Bioquímica
dc.subject.unesco3207 Patología
dc.titleDynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublication2ce1597c-df9d-46cd-b799-7b421378ee34
relation.isAuthorOfPublication.latestForDiscovery2ce1597c-df9d-46cd-b799-7b421378ee34

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