Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion

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dc.contributor.authorQuinchia, Jennifer
dc.contributor.authorBlázquez-García, Marina
dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorRejas-González, Raquel
dc.contributor.authorMontero-Calle, Ana
dc.contributor.authorOrozco, Jahir
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorCampuzano Ruiz, Susana
dc.date.accessioned2024-07-05T14:52:57Z
dc.date.available2024-07-05T14:52:57Z
dc.date.issued2024-01
dc.description.abstractDetecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H2O2/HRP) redox system. The method allows the selective detection of TIM-1 protein over the 87–7500 pg mL−1 concentration range in only 45 min, with a limit of detection of 26 pg mL−1. The developed bioplatform was successfully applied to the analysis of breast and lung cancer cell extracts, providing the first quantitative results of the target glycoprotein in these types of samples.
dc.description.departmentDepto. de Química Analítica
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Ciencias Químicas
dc.description.fundingtypeDescuento UCM
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationJennifer Quinchia, Marina Blázquez-García, Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Verónica Serafín, Raquel Rejas-González, Ana Montero-Calle, Jahir Orozco, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion, Talanta, Volume 267, 2024, 125155, ISSN 0039-9140,
dc.identifier.doi10.1016/j.talanta.2023.125155
dc.identifier.issn0039-9140
dc.identifier.officialurlhttps://doi.org/10.1016/j.talanta.2023.125155
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S0039914023009062?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/105710
dc.journal.titleTalanta
dc.language.isoeng
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu543
dc.subject.cdu577.1
dc.subject.keywordTIM-1
dc.subject.keywordAmperometric detection
dc.subject.keywordImmunoplatform
dc.subject.keywordScreen-printed carbon electrode
dc.subject.keywordMagnetic particles
dc.subject.keywordBreast and lung cancer
dc.subject.ucmCiencias
dc.subject.unesco2301 Química Analítica
dc.subject.unesco2302 Bioquímica
dc.titleDisposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number267
dspace.entity.typePublication
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