A comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance

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dc.contributor.authorLópez Ureña, Nadia María
dc.contributor.authorCalero Bernal, Rafael
dc.contributor.authorVázquez Calvo, Ángela
dc.contributor.authorSánchez Sánchez, Roberto
dc.contributor.authorOrtega Mora, Luis Miguel
dc.contributor.authorÁlvarez García, Gema
dc.date.accessioned2024-05-22T15:58:29Z
dc.date.available2024-05-22T15:58:29Z
dc.date.issued2023-10-03
dc.description2023 Acuerdos Transformativos CRUE
dc.description.abstractToxoplasma gondii is a major foodborne zoonotic pathogen that can be transmitted through the consumption of raw or undercooked meat of small ruminants, among others. Serology has been suggested as an epidemiological indicator and several tests are available nowadays. However, there is no comparative study with the most used ones. Therefore, the objective of this study was to develop and validate two in-house tests (Western blot -TgSALUVET WB- and ELISA -TgSALUVET ELISA 2.0-) and perform a comparative study including such tests and four commercial ELISA kits (IDScreen®, PrioCHECK®, Pigtype® and IDEXX). First, a specific pattern of recognition of immunodominant antigens by TgSALUVET WB was determined with serum panels of noninfected sheep and sheep infected with T. gondii or Neospora caninum. Next, TgSALUVET WB was used as a reference to preliminary validate TgSALUVET ELISA 2.0 using sera from sheep and goats naturally infected with T. gondii. Then, the abovementioned sheep serum panels were analyzed by all tests and subjected to TG-ROC analyses and agreement tests, and cross-reactivity with the anti-N. caninum IgGs was studied. All the techniques were accurate enough for the cutoff values initially suggested with all serum panels (Se and Sp ≥ 94%), except for PrioCHECK®, which showed 83% Sp. However, a cutoff readjustment improved their diagnostic performance. Additionally, cross-reactions between anti-N. caninum antibodies and T. gondii antigens were detected with all tests. Thus, a second cutoff readjustment was carried out and the use of both readjusted cutoff values is recommended to obtain comparable data and avoid false-positive results.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.facultyFac. de Veterinaria
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.sponsorshipBanco Santander
dc.description.statuspub
dc.identifier.citationLópez-Ureña, N. M., Calero-Bernal, R., Vázquez-Calvo, Á., Sánchez-Sánchez, R., Ortega-Mora, L. M., & Álvarez-García, G. (2023). A comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance. Research in veterinary science, 165, 105052. https://doi.org/10.1016/j.rvsc.2023.105052
dc.identifier.doi10.1016/j.rvsc.2023.105052
dc.identifier.essn1532-2661
dc.identifier.issn0034-5288
dc.identifier.officialurlhttps://doi.org/10.1016/j.rvsc.2023.105052
dc.identifier.pmid37864907
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/37864907/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104333
dc.issue.number105052
dc.journal.titleResearch in Veterinary Science
dc.language.isoeng
dc.page.final11
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/773830/EU
dc.relation.projectIDCT42/18
dc.relation.projectIDCT43/18
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu616.9:636.2/.3.09
dc.subject.keywordToxoplasma gondii
dc.subject.keywordELISA
dc.subject.keywordWestern blot
dc.subject.keywordSmall ruminants
dc.subject.keywordDiagnostic performance
dc.subject.keywordCross-reactions
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleA comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number165
dspace.entity.typePublication
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