Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant

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dc.contributor.authorCañadas Benito, Olga
dc.contributor.authorGarcía-García, Andrea
dc.contributor.authorPrieto, M. Auxiliadora
dc.contributor.authorPérez-Gil, Jesús
dc.date.accessioned2023-06-17T09:23:10Z
dc.date.available2023-06-17T09:23:10Z
dc.date.issued2021-06-03
dc.description.abstractPolyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/69937
dc.identifier.doi10.3390/nano11061482
dc.identifier.issn2079-4991
dc.identifier.officialurlhttps://doi.org/10.3390/nano11061482
dc.identifier.relatedurlhttps://www.mdpi.com/2079-4991/11/6/1482
dc.identifier.urihttps://hdl.handle.net/20.500.14352/8693
dc.issue.number6
dc.journal.titleNanomaterials
dc.language.isoeng
dc.page.final17
dc.page.initial1
dc.publisherMDPI
dc.relation.projectID(RTI2018-094564-B-I00, BIO2017-83448-R)
dc.relation.projectID(P2018/NMT-4389)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu577.112
dc.subject.cdu539.2:620.1
dc.subject.keywordDPPC
dc.subject.keywordPOPG
dc.subject.keywordPHA
dc.subject.keywordDynamic light scattering
dc.subject.keywordLipid monolayers
dc.subject.keywordRelaxation kinetics
dc.subject.keywordEpifluorescence microscopy
dc.subject.keywordpolyhydroxyalkanoates
dc.subject.keywordPHA nanoparticles
dc.subject.ucmBioquímica (Biología)
dc.subject.unesco2302 Bioquímica
dc.titlePolyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant
dc.typejournal article
dc.volume.number11
dspace.entity.typePublication
relation.isAuthorOfPublication25a89a2f-a381-4f15-9a6b-59430ee96a63
relation.isAuthorOfPublication.latestForDiscovery25a89a2f-a381-4f15-9a6b-59430ee96a63
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