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Volatile organo-selenium speciation in biological matter by solid phase microextraction–moderate temperature multicapillary gas chromatography with microwave induced plasma atomic emission spectrometry detection

dc.contributor.authorDietz, Christian
dc.contributor.authorSanz Landaluce, Jon
dc.contributor.authorXimenez Embun, Pilar
dc.contributor.authorMadrid Albarrán, María Yolanda
dc.contributor.authorCámara, Carmen
dc.date.accessioned2023-06-20T12:41:36Z
dc.date.available2023-06-20T12:41:36Z
dc.date.issued2004
dc.description.abstractMicrowave induced plasma atomic emission spectrometry (MIP-AES) in combination with multicapillary (MC) gas chromatography could be proven to be useful for element specific detection of volatile species. Solid phase microextraction (SPME) was used for preconcentration and sample-matrix separation. The fiber desorption unit as well as the heating control for the MCcolumn were in-house developed and multicapillary column was operated at moderate temperatures (30–100 ◦C). The method was optimized for organo-selenium species (dimethylselenide (DMSe), diethylselenide (DEtSe) and dimethyldiselenide (DMDSe)), using a chemometric approach. Stationary phases for the separation column were optimized using a conventional GC and contrasted with the results obtained with the MC. Application was focussed on selenium accumulating biological matter, such as lupine, yeast, Indian mustard and garlic. These samples were grown in hydroponic solution containing inorganic selenium (Na2SeO3 and Na2SeO4). SPME sampling was carried out in fixed volume flow boxes in headspace above the living plants and in vials using treated samples. Results demonstrate inorganic selenium transformation into volatile organic species during metabolism. Separation is fast, a chromatogram can be obtained in less than 3 min and detection limits were at sub-ppb level for all investigated species. The system is independent from the use of a conventional gas chromatographic oven and can be used as a versatile alternative to highly cost intensive methods such as GC–ICP-MS.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/4909
dc.identifier.issn0003-2670
dc.identifier.urihttps://hdl.handle.net/20.500.14352/52239
dc.journal.titleAnalitica Quimica Acta
dc.language.isoeng
dc.page.final167
dc.page.initial157
dc.rights.accessRightsopen access
dc.subject.keywordSolid phase microextraction
dc.subject.keywordMicrowave induced plasma emission spectroscopy
dc.subject.keywordOrgano-selenium compounds
dc.subject.keywordMulticapillary columns
dc.subject.keywordLupine
dc.subject.keywordYeast
dc.subject.keywordIndian mustard
dc.subject.keywordGarlic
dc.subject.keywordChemometrics
dc.subject.ucmMedio ambiente
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2391 Química Ambiental
dc.subject.unesco2301 Química Analítica
dc.titleVolatile organo-selenium speciation in biological matter by solid phase microextraction–moderate temperature multicapillary gas chromatography with microwave induced plasma atomic emission spectrometry detection
dc.typejournal article
dc.volume.number501
dspace.entity.typePublication
relation.isAuthorOfPublication085057ba-b26e-4219-93d5-5fcf1150fb20
relation.isAuthorOfPublication190287de-ed47-4954-86ff-d4f1eddff035
relation.isAuthorOfPublication.latestForDiscovery190287de-ed47-4954-86ff-d4f1eddff035

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