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TRPV4 activation triggers the release of melatonin from human non-pigmented ciliary epithelial cells

dc.contributor.authorAlkozi, Hanan Awad
dc.contributor.authorPintor, Jesús
dc.date.accessioned2023-06-18T05:41:36Z
dc.date.available2023-06-18T05:41:36Z
dc.date.issued2015-07
dc.descriptionReceived 11 December 2014; received in revised form 10 March 2015; accepted in revised form 27 April 2015; available online 29 April 2015.
dc.description.abstractMelatonin is a neurohormone mainly produced in the pineal gland; nevertheless, various ocular structures such as the ciliary body, lens and the retina produce it. One of the roles of melatonin in the eye is the modulation of intraocular pressure, although little is known about the mechanisms that causes its presence in the aqueous humour. TRPV4 is a membrane channel which is activated by both physical and chemical stimuli. Therefore, this channel is sensitive to osmotic and hydrostatic pressure. As a consequence, TRPV4 results as an interesting candidate to study the relation between the activation of the TRPV4 channel and the production of melatonin. In this sense we have studied the role of the TRPV4 agonist GSK1016790A to modulate the production of melatonin in a cell line derived from human non-pigmented ciliary epithelial cells. The stimulation of the TRPV4 produced an increase in the extracellular melatonin levels changing from 8.5 ± 0.6 nM/well/30 min (control) to 23.3 ± 2.1 nM/well/30 min after 10 nM GSK1016790A application, this action being blocked by the selective antagonist RN 1734. The activation of the TRPV4 by GSK1016790A permitted to observe a melatonin increase which was concentration-dependent, and provided a pD2 value of −8.5 ± 0.1 (EC50 of 3.0 nM). In conclusion, the activation of the TRPV4 present in human non-pigmented ciliary epithelial cells can modulate the presence of extracellular melatonin, this being of relevance since this substance controls the dynamics of the aqueous humour.
dc.description.departmentUnidad Docente de Bioquímica y Biología Molecular
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/40617
dc.identifier.doi10.1016/j.exer.2015.04.019
dc.identifier.issn0014-4835
dc.identifier.officialurlhttp://dx.doi.org/10.1016/j.exer.2015.04.019
dc.identifier.urihttps://hdl.handle.net/20.500.14352/23062
dc.journal.titleExperimental Eye Research
dc.language.isoeng
dc.page.final37
dc.page.initial34
dc.publisherElsevier
dc.relation.projectIDSAF2010-16024
dc.relation.projectIDSAF2013-44416-R
dc.relation.projectIDRETICS RD12/0034/0003
dc.relation.projectIDPR1/07-14890
dc.rights.accessRightsrestricted access
dc.subject.cdu617.725
dc.subject.cdu617.749
dc.subject.cdu615.357
dc.subject.keywordAqueous humour
dc.subject.keywordGSK1016790A
dc.subject.keywordMelatonin
dc.subject.keywordRN 1734
dc.subject.keywordTRPV4
dc.subject.ucmBioquímica (Medicina)
dc.subject.ucmOftalmología
dc.subject.ucmAnatomía ocular
dc.subject.unesco3201.09 Oftalmología
dc.titleTRPV4 activation triggers the release of melatonin from human non-pigmented ciliary epithelial cells
dc.typejournal article
dc.volume.number136
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