Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles

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dc.contributor.authorVillalonga, María
dc.contributor.authorBorisova, Boryana
dc.contributor.authorArenas, Christian
dc.contributor.authorVillalonga, Anabel
dc.contributor.authorArévalo-Villena, María
dc.contributor.authorSánchez Sánchez, Alfredo
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorBriones-Pérez, Ana
dc.contributor.authorVillalonga Santana, Reynaldo
dc.date.accessioned2024-01-12T09:26:52Z
dc.date.available2024-01-12T09:26:52Z
dc.date.issued2018
dc.description.abstractTwo different disposable amperometric biosensors for the rapid detection and quantification of Brettanomyces bruxellensis (Brett) and total yeast content in wine were constructed. The biosensing approach implied the use of magnetic capturing elements based on core-shell Fe3O4@SiO2 superparamagnetic nanoparticles (NanoCaptors) functionalized with specific bioreceptors: Concanavalin A (Con A) for total yeast analysis, and anti-Brett polyclonal antibodies for Brett. A Con A-peroxidase conjugate and carbon screen printed electrodes were used as signaling element and sensing interfaces, respectively. The biosensor based on antibody-modified magnetic nanoparticles allowed the amperometric detection of B. bruxellensis in buffer solution and red wine samples in the range of 10 - 106 CFU/mL, with low detection limits of 6 CFU/mL and 8 CFU/mL, respectively. In addition, the use of Con A-linked magnetic nanoparticles as capturing elements allowed determination of total yeast content in buffer solution and red wine in the range of 10 - 106 CFU/mL, with detection limit of 5 CFU/mL. These electrochemical biosensors also exhibited high reproducibility, selectivity and storage stability.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economia y ́Competitividad (España)
dc.description.sponsorshipJunta de Comunidades de Castilla La Mancha
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationVillalonga, M. L., Borisova, B., Arenas, C. B., Villalonga, A., Arévalo-Villena, M., Sánchez, A., Pingarrón, J. M., Briones-Pérez. A., Villalonga, R.* (2019). Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles. Sensors and Actuators B: Chemical, 279, 15-21.
dc.identifier.doi10.1016/j.snb.2018.09.092
dc.identifier.issn0925-4005
dc.identifier.officialurlhttps://doi.org/10.1016/j.snb.2018.09.092
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92685
dc.journal.titleSensors & Actuators: B. Chemical
dc.language.isoeng
dc.page.final21
dc.page.initial15
dc.publisherElsevier
dc.relation.projectID(MINECO Projects CTQ2014-58989-P, CTQ2015- 71936-REDT and CTQ2017-87954-P)
dc.relation.projectID(JCCM Project POII-2014-011-A)
dc.relation.projectIDNANOAVANSENS (Project S2013/ MIT-3029)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu543
dc.subject.keywordBrettanomyces bruxellensis
dc.subject.keywordSaccharomyces cerevisiae
dc.subject.keywordBiosensor
dc.subject.keywordYeast
dc.subject.keywordRed wine
dc.subject.keywordScreen printed electrodes
dc.subject.ucmCiencias
dc.subject.unesco23 Química
dc.titleDisposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number279
dspace.entity.typePublication
relation.isAuthorOfPublication7b70b8e0-9e04-4e8d-887d-0981d2309664
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublicationa681d857-88a1-4eb9-ab7d-c411c59b3f80
relation.isAuthorOfPublication.latestForDiscovery7b70b8e0-9e04-4e8d-887d-0981d2309664
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