Stability characteristics of cannabidiol for the design of pharmacological, biochemical and pharmaceutical studies

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dc.contributor.authorFraguas Sánchez, Ana Isabel
dc.contributor.authorFernández Carballido, Ana María
dc.contributor.authorMartín Sabroso, Cristina
dc.contributor.authorTorres Suárez, Ana Isabel
dc.date.accessioned2024-01-12T09:26:13Z
dc.date.available2024-01-12T09:26:13Z
dc.date.issued2020-08
dc.description.abstractCannabidiol (CBD) is one of the most promising cannabinoids in therapeutics. Nevertheless, the reported stability testing has been carried out with plant extracts and not with CBD as a drug substance. The aim of this work was to evaluate the stability of CBD in solution. A High-Performance Liquid Chromatography (HPLC) analytical method, with CBD in ethanol, was previously validated for these stability studies. The resulting method was linear and proportional in a range of concentrations from 1 to 150 µg CBD/mL, as well as precise. It was also considered suitable to quantify CBD in aqueous medium as reported in accuracy studies. The stability of CBD was influenced by multiple factors. Temperature was one of the most critical parameters, with an activation energy of 92.19KJ/mol. At room temperature, CBD was highly unstable (t95 = 117.13 days). However, at 5 °C it was stable for at least 12 months. CBD was also sensitive to oxidation, with a short t95 of 1.77 days in oxidizing environments, as well as to light. The photolytic reaction seems to be oxidative. The solvent influences CBD stability, and the latter is more stable in ethanol than in aqueous medium. In fact, in simulated physiological conditions (pH 7.4 and 37 °C) 10% of CBD was degraded within 24 h. These studies indicate that CBD is highly unstable, and this should be taken into account in the development of in vitro and in vivo studies of CBD activity and in the pharmaceutical development of dosage forms.
dc.description.departmentDepto. de Farmacia Galénica y Tecnología Alimentaria
dc.description.facultyFac. de Farmacia
dc.description.facultyInstituto Universitario de Farmacia Industrial
dc.description.refereedTRUE
dc.description.sponsorshipComplutense Research Fund
dc.description.sponsorshipSpanish Ministry of Education
dc.description.sponsorshipSantander-UCM research group “Formulation and bioavailability of new drug products”
dc.description.statuspub
dc.identifier.citationFraguas-Sánchez AI, Fernández-Carballido A, Martin-Sabroso C, Torres-Suárez AI. Stability characteristics of cannabidiol for the design of pharmacological, biochemical and pharmaceutical studies. Journal of Chromatography B 2020;1150:122188. https://doi.org/10.1016/j.jchromb.2020.122188
dc.identifier.doi10.1016/j.jchromb.2020.122188
dc.identifier.essn1873-376X
dc.identifier.issn1570-0232
dc.identifier.officialurlhttps://doi.org/10.1016/j.jchromb.2020.122188
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92683
dc.journal.titleJournal of chromatography B
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/FPU14/06441
dc.relation.projectIDinfo:eu-repo/grantAgreement/FEI16/83
dc.relation.projectIDinfo:eu-repo/grantAgreement/UCM-910939
dc.rights.accessRightsrestricted access
dc.subject.cdu615.4
dc.subject.keywordCannabidiol
dc.subject.keyworddegradation
dc.subject.keywordHPLC
dc.subject.keywordself-life
dc.subject.keywordstability
dc.subject.keywordoxidation
dc.subject.ucmTecnología farmaceútica
dc.subject.unesco3209.08 Preparación de Medicamentos
dc.titleStability characteristics of cannabidiol for the design of pharmacological, biochemical and pharmaceutical studies
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1150
dspace.entity.typePublication
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