Assessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification

Simple item page
dc.contributor.authorPérez Sancho, Marta
dc.contributor.authorVela Alonso, Ana Isabel
dc.contributor.authorGottschalk, Marcelo
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.contributor.authorGarcía-Seco Romero, María Teresa
dc.date.accessioned2023-06-19T15:04:20Z
dc.date.available2023-06-19T15:04:20Z
dc.date.issued2015
dc.description.abstractThe accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. After updating the currently available S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7, and 9, most isolates had statistically significant higher score values (mean score: 2.65) than those obtained using the original database (mean score: 2.182). Considering the results of the present study, we suggest using a less restrictive threshold score of ≥2.000 for reliable species identification of S. suis. According to this cut-off value, a total of 125 S. suis isolates (96.9%) were correctly identified using the updated database. These data indicate an excellent performance of MALDI-TOF MS for the identification of S. suis.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipMoncloa: Campus de Excelencia Internacional
dc.description.sponsorshipMinisterio de Educación, Cultura y Deporte
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39618
dc.identifier.doi10.3389/fpubh.2015.00202
dc.identifier.issn2296-2565
dc.identifier.officialurlhttps://doi.org/10.3389/fpubh.2015.00202
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35267
dc.journal.titleFrontiers in Public Health
dc.language.isoeng
dc.page.initial202
dc.publisherFrontiers Media
dc.relation.projectID(Programa CEI09-0019)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordIdentification
dc.subject.keywordMALDI-TOF MS
dc.subject.keywordStreptococcus suis
dc.subject.keywordPCR
dc.subject.keywordbiochemical tests
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleAssessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification
dc.typejournal article
dc.volume.number3
dspace.entity.typePublication
relation.isAuthorOfPublication94b7d8ef-e913-46ee-beeb-2d37b3caeb7f
relation.isAuthorOfPublication83481d48-83ea-472f-8b74-067bc00debee
relation.isAuthorOfPublication5d5c3513-e6d9-451a-ba7d-ba0634c84bcf
relation.isAuthorOfPublicationbaae9555-7eff-4de2-88b8-ad1c8bf8dd98
relation.isAuthorOfPublication2afbcaa4-1eb6-44e1-bbcd-808c842cb8f7
relation.isAuthorOfPublication.latestForDiscovery94b7d8ef-e913-46ee-beeb-2d37b3caeb7f
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
772.pdf
Size:
652.17 KB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos