Pulmonary surfactant protein A-mediated enrichment of surface-decorated polymeric nanoparticles in alveolar macrophages

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dc.contributor.authorRuge, Christian
dc.contributor.authorHillaireau, Hervé
dc.contributor.authorGrabowski, Nadège
dc.contributor.authorBeck-Broichsitter, Mortiz
dc.contributor.authorCañadas Benito, Olga
dc.contributor.authorTsapis, Nicolas
dc.contributor.authorCasals Carro, María Cristina
dc.contributor.authorNicolas, Julien
dc.contributor.authorFattal, Elias
dc.date.accessioned2024-01-22T15:10:02Z
dc.date.available2024-01-22T15:10:02Z
dc.date.issued2016
dc.description.abstractSurfactant protein A (SP-A), a lung anti-infective protein, is a lectin with affinity for sugars found on fungal and micrococcal surfaces such as mannose. We synthesized a mannosylated poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) copolymer and used it to produce nanoparticles with a polyester (PLGA/PLA) core and a PEG shell decorated with mannose residues, designed to be strongly associated with SP-A for an increased uptake by alveolar macrophages. Nanoparticles made of the copolymers were obtained by nanoprecipitation and displayed a size of around 140 nm. The presence of mannose on the surface was demonstrated by zeta potential changes according to pH and by a strong aggregation in the presence of concanavalin A. Mannosylated nanoparticles bound to SP-A as demonstrated by dynamic light scattering and transmission electron microscopy. The association with SP-A increased nanoparticle uptake by THP-1 macrophages in vitro. In vivo experiments demonstrated that after intratracheal administration of nanoparticles with or without SP-A, SP-A-coated mannosylated nanoparticles were internalized by alveolar macrophages in greater proportion than SP-A-coated nonmannosylated nanoparticles. The data demonstrate for the first time that the pool of nanoparticles available to lung cells can be changed after surface modification, using a biomimetic approach.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipGerman Research Foundation
dc.description.statuspub
dc.identifier.citationRuge C, Hillaireau H, Grabowski N, Beck-Broichsitter M, Cañadas O, Tsapis N, Casals C, Nicolas J, Fattal E. Pulmonary surfactant protein A – mediated enrichment of surface-decorated polymeric nanoparticles in alveolar macrophages. Mol. Pharmaceutics. 2016 Dec 5;13(12): 4168-4178
dc.identifier.doi10.1021/acs.molpharmaceut.6b00773
dc.identifier.essn1543-8392
dc.identifier.issn1543-8384
dc.identifier.officialurlhttps://doi.org/10.1021/acs.molpharmaceut.6b00773
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94444
dc.issue.number12
dc.journal.titleMolecular Pharmaceutics
dc.language.isoeng
dc.page.final4178
dc.page.initial4168
dc.publisherAmerican Chemical Society
dc.relation.projectID(RU 1866/1-2)
dc.rights.accessRightsrestricted access
dc.subject.cdu577.112
dc.subject.cdu616.2
dc.subject.cdu615.4
dc.subject.keywordLungs
dc.subject.keywordMacrophages
dc.subject.keywordMannose
dc.subject.keywordNanoparticles
dc.subject.keywordSurfactant protein A
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmBiología molecular (Química)
dc.subject.ucmFarmacología (Medicina)
dc.subject.unesco2302.21 Biología Molecular
dc.subject.unesco2302.22 Farmacología Molecular
dc.subject.unesco2403 Bioquímica
dc.titlePulmonary surfactant protein A-mediated enrichment of surface-decorated polymeric nanoparticles in alveolar macrophages
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number13
dspace.entity.typePublication
relation.isAuthorOfPublication25a89a2f-a381-4f15-9a6b-59430ee96a63
relation.isAuthorOfPublicationd4e23d80-fa5c-4614-bd2d-2c391b596713
relation.isAuthorOfPublication.latestForDiscovery25a89a2f-a381-4f15-9a6b-59430ee96a63
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