Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017

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dc.contributor.authorGarcía Benzaquén, Nerea
dc.contributor.authorMarta Hernández
dc.contributor.authorMaialen Gutierrez-Boada
dc.contributor.authorAntonio Valero
dc.contributor.authorNavarro Gómez, Alejandro
dc.contributor.authorMilagros Muñoz-Chimeno
dc.contributor.authorFernández Manzano, Álvaro
dc.contributor.authorFranco Matías Escobar
dc.contributor.authorMartínez Alares, Irene
dc.contributor.authorBárcena Asensio, María Carmen
dc.contributor.authorGonzález Domínguez, Sergio
dc.contributor.authorAna Avellón
dc.contributor.authorJose M. Eiros
dc.contributor.authorGislaine Fongaro
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorGoyache Goñi, Joaquín
dc.contributor.authorDavid Rodríguez-Lázaro
dc.date.accessioned2024-01-15T13:42:33Z
dc.date.available2024-01-15T13:42:33Z
dc.date.issued2020-01-28
dc.descriptionThis study was partially supported by the RTA2014-00024-C04 from the Spanish Ministry of Economy and Innovation.
dc.description.abstractZoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from nine Spanish slaughterhouses (50% national production) that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by reverse transcription realtime PCR (RT-qPCR). The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed, a total of 26 RT-qPCR positive samples were identified in the liver (7/45), feces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham, and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (RTA2014-00024-C04)
dc.description.statuspub
dc.identifier.citationGarcía N, Hernández M, Gutierrez-Boada M, Valero A, Navarro A, Muñoz-Chimeno M, Fernández-Manzano A, Escobar FM, Martínez I, Bárcena C, González S, Avellón A, Eiros JM, Fongaro G, Domínguez L, Goyache J and Rodríguez-Lázaro D (2020) Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017. Front. Microbiol. 10:2990. doi: 10.3389/fmicb.2019.02990
dc.identifier.doi10.3389/fmicb.2019.02990
dc.identifier.issn2297-1769
dc.identifier.officialurlhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02990/full
dc.identifier.relatedurlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997137/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93121
dc.journal.titleFrontiers in Veterinary Science
dc.language.isoeng
dc.page.initial2990
dc.publisherFRONTIERS MEDIA SA
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordfood safety;
dc.subject.keywordhepatitis E virus
dc.subject.keywordPig organs
dc.subject.keywordPigs
dc.subject.keywordPork cuttings
dc.subject.keywordPrevalence
dc.subject.keywordSeroprevalence
dc.subject.keywordSlaughterhouse
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleOccurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number10
dspace.entity.typePublication
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