HPLC isolation of antioxidant constituents from Xanthoparmelia spp

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dc.contributor.authorAmo De Paz, Guillermo
dc.contributor.authorRaggio Quílez, José
dc.contributor.authorGómez-Serranillos Cuadrado, María Pilar
dc.contributor.authorPalomino Ruiz-Poveda, Olga María
dc.contributor.authorGonzález Burgos, Elena María
dc.contributor.authorCarretero Accame, María Emilia
dc.contributor.authorCrespo De Las Casas, Ana María
dc.date.accessioned2024-01-12T09:19:23Z
dc.date.available2024-01-12T09:19:23Z
dc.date.issued2010-04-24
dc.description.abstractA chromatographic method is described for the purification and characterization of secondary lichen substances with biological activity. A simple reversed-phase high-performance liquid chromatography method with gradient elution has been developed that allows the determination and isolation of salazinic, usnic and stictic acids from lichen samples in a single run and the quantification of every acid in the tested extracts. The antioxidant activity of both the isolated compounds and the respective lichen belonging to Xanthoparmelia genus was determined by the Oxygen Radical Absorbance Capacity (ORAC) assay; their effect as free radical scavengers, effect on cell survival by the 3(4,5-dimethyltiazol-2-yl)-2,5-diphenyltetrazolium reduction assay and 2′,7′-dichlorofluorescin diacetate method were tested on U373 MG human astrocytome cell line. Both lichens extracts and all isolated compounds protected U373 MG cells from hydrogen peroxide-induced damage, suggesting that they could act as antioxidant agents in those neurodegenerative disorders associated with oxidative damage, such as Alzheimer's disease and Parkinson's disease.
dc.description.departmentDepto. de Farmacología, Farmacognosia y Botánica
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationAmo de Paz, G.; Raggio, J.; Gómez-Serranillos, M.P.; Palomino, O.M.; González-Burgos, E.; Carretero, M.E.; Crespo, A. HPLC isolation of antioxidant constituents from Xanthoparmelia spp. J. Pharm. Biomed. Anal. 2010, 53, 165–171
dc.identifier.doi10.1016/j.jpba.2010.04.013
dc.identifier.essn1873-264X
dc.identifier.issn0731-7085
dc.identifier.officialurlhttps://doi.org/10.1016/j.jpba.2010.04.013
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92680
dc.issue.number2
dc.journal.titleJournal of Pharmaceutical and Biomedical Analysis
dc.language.isoeng
dc.page.final171
dc.page.initial165
dc.publisherElsevier
dc.rights.accessRightsrestricted access
dc.subject.cdu664.022
dc.subject.cdu615.212
dc.subject.keywordHPLC
dc.subject.keywordsalazinic acid
dc.subject.keywordusnic acid
dc.subject.keywordstictic acid
dc.subject.keywordantioxidant
dc.subject.keywordastrocyte
dc.subject.ucmFarmacología (Farmacia)
dc.subject.ucmFisiología vegetal (Biología)
dc.subject.unesco2417.19 Fisiología Vegetal
dc.subject.unesco3209.05 Farmacognosia
dc.titleHPLC isolation of antioxidant constituents from Xanthoparmelia spp
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number53
dspace.entity.typePublication
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