Arsenate and arsenite differential toxicity in Tetrahymena thermophila

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dc.contributor.authorRodriguez Martín, Daniel
dc.contributor.authorMurciano Cespedosa, Antonio
dc.contributor.authorHerráiz Moreno, Marta
dc.contributor.authorDe Francisco Martínez, Patricia
dc.contributor.authorAmaro Torres, Francisco
dc.contributor.authorGutiérrez Fernández, Juan Carlos
dc.contributor.authorMartín-González, Ana María
dc.contributor.authorDíaz Del Toro, Silvia
dc.date.accessioned2023-06-22T10:43:26Z
dc.date.available2023-06-22T10:43:26Z
dc.date.issued2022-02-25
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2022)
dc.description.abstractA comparative analysis of toxicities of both arsenic forms (arsenite and arsenate) in the model eukaryotic microorganism Tetrahymena thermophila (ciliate protozoa) has shown the presence of various detoxification mechanisms and cellular effects comparable to those of animal cells under arsenic stress. In the wild type strain SB1969 arsenate is almost 2.5 times more toxic than arsenite. According to the concentration addition model used in binary metallic mixtures their toxicities show an additive effect. Using fluorescent assays and flow cytometry, it has been detected that As(V) generates elevated levels of ROS/RNS compared to As(III). Both produce the same levels of superoxide anion, but As(V) also causes greater increases in hydrogen peroxide and peroxynitrite. The mitochondrial membrane potential is affected by both As(V) and As(III), and electron microscopy has also revealed that mitochondria are the main target of both arsenic ionic forms. Fusion/fission and swelling mitochondrial and mitophagy, together with macroautophagy, vacuolization and mucocyst extruction are mainly associated to As(V) toxicity, while As(III) induces an extensive lipid metabolism dysfunction (adipotropic effect). Quantitative RT-PCR analysis of some genes encoding antioxidant proteins or enzymes has shown that glutathione and thioredoxin metabolisms are involved in the response to arsenic stress. Likewise, the function of metallothioneins seems to be crucial in arsenic detoxification processes, after using both metallothionein knockout and knockdown strains and cells overexpressing metallothionein genes from this ciliate. The analysis of the differential toxicity of As(III) and As(V) shown in this study provides cytological and molecular tools to be used as biomarkers for each of the two arsenic ionic forms.en
dc.description.departmentDepto. de Biodiversidad, Ecología y Evolución
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/72493
dc.identifier.doi10.1016/j.jhazmat.2022.128532
dc.identifier.issn0304-3894
dc.identifier.officialurlhttps://doi.org/10.1016/j.jhazmat.2022.128532
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S030438942200320X
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71508
dc.issue.number128532
dc.journal.titleJournal of Hazardous Materials
dc.language.isoeng
dc.page.final16
dc.page.initial1
dc.publisherElsevier
dc.relation.projectID(CGL2016-75494-R)
dc.rightsAttribution 4.0 International
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu593.17
dc.subject.cdu579.25
dc.subject.cdu546.19
dc.subject.keywordTetrahymena
dc.subject.keywordArsenite and arsenate
dc.subject.keywordToxicity
dc.subject.keywordMitochondrial dysfunction
dc.subject.keywordMetallothioneins
dc.subject.keywordAntioxidant gene expression
dc.subject.ucmToxicología (Medicina)
dc.subject.ucmInvertebrados
dc.subject.ucmMicrobiología (Biología)
dc.subject.ucmGenética
dc.subject.unesco3214 Toxicología
dc.subject.unesco2401.17 Invertebrados
dc.subject.unesco2414 Microbiología
dc.subject.unesco2409 Genética
dc.titleArsenate and arsenite differential toxicity in Tetrahymena thermophila
dc.typejournal article
dc.volume.number431
dspace.entity.typePublication
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relation.isAuthorOfPublication469a6187-001d-4d49-a795-9072ff43f702
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relation.isAuthorOfPublication.latestForDiscoverya08bc152-a727-4918-8e7e-1fab4ad77e8f
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