Autohydrolysis and microwave ionic liquid pretreatment of Pinus radiata: Imaging visualization and analysis to understand enzymatic digestibility

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dc.contributor.authorRigual Hernández, Victoria De Los Ángeles
dc.contributor.authorDomínguez Toribio, Juan Carlos
dc.contributor.authorSantos, Tamara
dc.contributor.authorRivas Siota, Sandra
dc.contributor.authorAlonso Rubio, María Virginia
dc.contributor.authorOliet Pala, María Mercedes
dc.contributor.authorRodriguez, Francisco
dc.date.accessioned2024-02-02T13:13:35Z
dc.date.available2024-02-02T13:13:35Z
dc.date.issued2019
dc.description.abstractSequential pretreatments provide advantages of every single process towards a complete biomass fractionation. In this work, autohydrolysis and IL microwave pretreatments are sequentialy studied, and their effect to enhance enzymatic hydrolysis is analyzed. Mild, intermediate and severe autohydrolysis are combined with four IL temperatures (50, 80, 120 and 150 °C). Pretreated solids are enzymatically hydrolyzed and compared in terms of chemical composition and morphology. Digital image analysis is employed to numerically determine the heterogeneity of the solids using surface fractal dimension and lacunarity parameters. In this study, the negative effect of intermediate and severe autohydrolysis, over the subsequent IL pretreatment is demonstrated. Mild autohydrolysis and high IL conditions (AH150IL120) results in digestibilities of 78.8 g of glucan/100 g of glucan introduced. High surface fractal values (in the range of 2.5461–2.7124) and low lacunarities (0.0818–0.2563) enhance the enzymatic accessibility of pine wood. Furthermore, the negative effect of softwood lignin accumulation in the surface is observed using confocal fluorescence microscopy.
dc.description.departmentDepto. de Ingeniería Química y de Materiales
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.identifier.citationRigual, Victoria, et al. «Autohydrolysis and Microwave Ionic Liquid Pretreatment of Pinus Radiata: Imaging Visualization and Analysis to Understand Enzymatic Digestibility». Industrial Crops and Products, vol. 134, agosto de 2019, pp. 328-37. https://doi.org/10.1016/j.indcrop.2019.03.068.
dc.identifier.doi10.1016/j.indcrop.2019.03.068
dc.identifier.issn0926-6690
dc.identifier.officialurlhttps://doi.org/10.1016/j.indcrop.2019.03.068
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98311
dc.journal.titleIndustrial Crops & Products
dc.language.isoeng
dc.page.final337
dc.page.initial328
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordAutohydrolysis, Ionic liquid, Surface fractal dimension, Enzymatic hydrolysis
dc.subject.ucmCiencias
dc.subject.unesco33 Ciencias Tecnológicas
dc.titleAutohydrolysis and microwave ionic liquid pretreatment of Pinus radiata: Imaging visualization and analysis to understand enzymatic digestibility
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number134
dspace.entity.typePublication
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