Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2
dc.contributor.author | Ruiz Valdepeñas Montiel, Víctor | |
dc.contributor.author | Pellicanò, Alessandro | |
dc.contributor.author | Campuzano Ruiz, Susana | |
dc.contributor.author | Torrente Rodríguez, Rebeca Magnolia | |
dc.contributor.author | Reviejo García, Ángel Julio | |
dc.contributor.author | Cosio, Maria Stella | |
dc.contributor.author | Pingarrón Carrazón, José Manuel | |
dc.date.accessioned | 2024-01-24T09:44:37Z | |
dc.date.available | 2024-01-24T09:44:37Z | |
dc.date.issued | 2016 | |
dc.description.abstract | A highly sensitive disposable amperometric magnetoimmunosensor for the rapid determination of Ara h 2 protein, one of the major peanut allergens, is reported. The approach uses a sandwich configuration involving selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Detector antibodies are labeled with HRP-conjugated secondary antibodies and the MBs bearing the immunoconjugates are magnetically captured on surface of a disposable screen-printed carbon electrode (SPCE). The affinity reactions are monitored amperometrically at −0.20 V (vs a Ag pseudo-reference electrode) in the presence of hydroquinone (HQ) as electron transfer mediator and upon addition of hydrogen peroxide as the enzyme substrate. The developed magnetoimmunosensor exhibits a wide range of linearity between 87 and 10,000 pg/mL Ara h 2 with a detection limit of 26 pg/mL as well as a great selectivity against other non-target proteins. The magnetoimmunosensing platform was successfully applied for the detection of Ara h 2 in different food extracts. After an appropriate sample dilution no matrix effects were observable. The developed methodology was able to detect trace amounts of the peanut allergen (0.0005% or 5.0 mg/kg) in wheat flour spiked samples. The results correlated properly with those provided by a commercial ELISA kit. | |
dc.description.department | Depto. de Química Analítica | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Economía y Competitividad (España) | |
dc.description.sponsorship | Comunidad de Madrid | |
dc.description.sponsorship | Ministry of Education (Italy) | |
dc.description.status | pub | |
dc.identifier.citation | Montiel, V. R. V., Pellicano, A., Campuzano, S., Torrente-Rodríguez, R. M., Reviejo, Á. J., Cosio, M. S., & Pingarrón, J. M. (2016). Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2. Sensors and Actuators B: Chemical, 236, 825-833. | |
dc.identifier.doi | 10.1016/j.snb.2016.01.123 | |
dc.identifier.issn | 0925-4005 | |
dc.identifier.officialurl | https://doi.org/10.1016/j.snb.2016.01.123 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/94995 | |
dc.journal.title | Sensors and Actuators B: Chemical | |
dc.language.iso | eng | |
dc.page.final | 833 | |
dc.page.initial | 825 | |
dc.publisher | Elsevier Science | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2015-64402-C2-1-R/ES/NUEVAS PLATAFORMAS DE MULTIPLEXADO ELECTROANALITICAS PARA LA DETECCION Y PRONOSTICO DE ENFERMEDADES NEOPLASICAS MEDIANTE BIOPSIAS LIQUIDAS/ | |
dc.relation.projectID | S2013/MT-3029 | |
dc.relation.projectID | MIUR, 2010 AXENJ8_002 | |
dc.rights.accessRights | restricted access | |
dc.subject.cdu | 543 | |
dc.subject.keyword | Ara h 2 | |
dc.subject.keyword | Magnetic beads | |
dc.subject.keyword | Screen-printed electrode | |
dc.subject.keyword | Amperometric immunosensor | |
dc.subject.keyword | Food extracts | |
dc.subject.ucm | Química | |
dc.subject.unesco | 2301 Química Analítica | |
dc.title | Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2 | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 236 | |
dspace.entity.type | Publication | |
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relation.isAuthorOfPublication | 1c26f036-cf31-4b55-9cab-f895e6789cae | |
relation.isAuthorOfPublication | 8808f99c-5f56-4562-839a-517626c76dad | |
relation.isAuthorOfPublication.latestForDiscovery | f7757d94-e7fd-4416-9a9f-4c2539dc2fd4 |
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