Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2

Simple item page
dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorPellicanò, Alessandro
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorReviejo García, Ángel Julio
dc.contributor.authorCosio, Maria Stella
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-24T09:44:37Z
dc.date.available2024-01-24T09:44:37Z
dc.date.issued2016
dc.description.abstractA highly sensitive disposable amperometric magnetoimmunosensor for the rapid determination of Ara h 2 protein, one of the major peanut allergens, is reported. The approach uses a sandwich configuration involving selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Detector antibodies are labeled with HRP-conjugated secondary antibodies and the MBs bearing the immunoconjugates are magnetically captured on surface of a disposable screen-printed carbon electrode (SPCE). The affinity reactions are monitored amperometrically at −0.20 V (vs a Ag pseudo-reference electrode) in the presence of hydroquinone (HQ) as electron transfer mediator and upon addition of hydrogen peroxide as the enzyme substrate. The developed magnetoimmunosensor exhibits a wide range of linearity between 87 and 10,000 pg/mL Ara h 2 with a detection limit of 26 pg/mL as well as a great selectivity against other non-target proteins. The magnetoimmunosensing platform was successfully applied for the detection of Ara h 2 in different food extracts. After an appropriate sample dilution no matrix effects were observable. The developed methodology was able to detect trace amounts of the peanut allergen (0.0005% or 5.0 mg/kg) in wheat flour spiked samples. The results correlated properly with those provided by a commercial ELISA kit.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinistry of Education (Italy)
dc.description.statuspub
dc.identifier.citationMontiel, V. R. V., Pellicano, A., Campuzano, S., Torrente-Rodríguez, R. M., Reviejo, Á. J., Cosio, M. S., & Pingarrón, J. M. (2016). Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2. Sensors and Actuators B: Chemical, 236, 825-833.
dc.identifier.doi10.1016/j.snb.2016.01.123
dc.identifier.issn0925-4005
dc.identifier.officialurlhttps://doi.org/10.1016/j.snb.2016.01.123
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94995
dc.journal.titleSensors and Actuators B: Chemical
dc.language.isoeng
dc.page.final833
dc.page.initial825
dc.publisherElsevier Science
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//CTQ2015-64402-C2-1-R/ES/NUEVAS PLATAFORMAS DE MULTIPLEXADO ELECTROANALITICAS PARA LA DETECCION Y PRONOSTICO DE ENFERMEDADES NEOPLASICAS MEDIANTE BIOPSIAS LIQUIDAS/
dc.relation.projectIDS2013/MT-3029
dc.relation.projectIDMIUR, 2010 AXENJ8_002
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordAra h 2
dc.subject.keywordMagnetic beads
dc.subject.keywordScreen-printed electrode
dc.subject.keywordAmperometric immunosensor
dc.subject.keywordFood extracts
dc.subject.ucmQuímica
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number236
dspace.entity.typePublication
relation.isAuthorOfPublicationf7757d94-e7fd-4416-9a9f-4c2539dc2fd4
relation.isAuthorOfPublication6945fb1e-9c48-459d-b994-e64328c3e79b
relation.isAuthorOfPublicationf4569700-ddad-4754-a155-145a8107c215
relation.isAuthorOfPublication1c26f036-cf31-4b55-9cab-f895e6789cae
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscoveryf7757d94-e7fd-4416-9a9f-4c2539dc2fd4
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Electrochemical_detection_of_peanuts.pdf
Size:
1.46 MB
Format:
Adobe Portable Document Format
Download
Collections
Artículos