Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study

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dc.contributor.authorMarín Cuenda, María José
dc.contributor.authorAmbrosio Elejalde, Nagore
dc.contributor.authorVirto Ruiz, Leire
dc.contributor.authorDiz, Pedro
dc.contributor.authorÁlvarez, Maximiliano
dc.contributor.authorHerrera, David
dc.contributor.authorSanz Alonso, Mariano
dc.contributor.authorFiguero Ruiz, Elena
dc.date.accessioned2024-01-24T11:39:16Z
dc.date.available2024-01-24T11:39:16Z
dc.date.issued2017-02-01
dc.description.abstractBackground: Culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. More recently, quantitative polymerase chain reaction (qPCR) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. Objective: To compare the ability to detect and quantify Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis (alone or in combination) in blood samples with three culture techniques [direct anaerobic culturing (DAC), haemo-culture (BACTEC), and lysis-centrifugation (LC)] and a non-culture dependent approach (qPCR) in an in vitro study. Material and methods: Blood samples from 12 periodontally healthy volunteers were contaminated with three concentrations [104,102 and 101 colony forming units (CFU)/mL] of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination. Samples were analysed by DAC, BACTEC, LC and qPCR. Sensitivity, specificity, predictive values, kappa index and Lińs correlation coefficients were calculated. Results: DAC, LC and qPCR were able to detect the three target species at all concentrations. An excellent concordance (correlation coefficient r: 0.92-1) was observed between DAC and the reference standard (sensitivity raging 93.33-100% and specificity 88.89-100%) values. BACTEC was not able to identify P. gingivalis in any of the performed experiments. qPCR provided false negative results for S.oralis. Conclusions: DAC showed the best results for the proper identification and quantification of A. actinomycetemcomitans, P. gingivalis and S. oralis, alone or in combination, in blood samples.
dc.description.departmentDepto. de Especialidades Clínicas Odontológicas
dc.description.facultyFac. de Odontología
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationMarin MJ, Ambrosio N, Virto L, Diz P, Álvarez M, Herrera D, Sanz M, Figuero E. Detection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study. Arch Oral Biol. 2017 Feb;74:55-62
dc.identifier.doi10.1016/j.archoralbio.2016.11.007
dc.identifier.essn1879-1506
dc.identifier.issn0003-9969
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0003996916303302?via%3Dihub
dc.identifier.pmid27875793
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95059
dc.journal.titleArchives of Oral Biology
dc.language.isoeng
dc.page.final62
dc.page.initial55
dc.publisherElsevier
dc.relation.projectIDFIS [PI11/00542]
dc.rights.accessRightsrestricted access
dc.subject.cdu616.314
dc.subject.cdu616.314.17-008.1
dc.subject.cdu579.61
dc.subject.keywordAggregatibacter actinomycetemitans
dc.subject.keywordBacteremia
dc.subject.keywordCulture
dc.subject.keywordPeriodontal
dc.subject.keywordPorphyromonas gingivalis
dc.subject.keywordQuantitative PCR
dc.subject.ucmOdontología (Odontología)
dc.subject.ucmPeriodoncia
dc.subject.ucmMicrobiología médica
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco3213.13 Ortodoncia-Estomatología
dc.subject.unesco3201.03 Microbiología Clínica
dc.titleDetection and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Streptococcus oralis in blood samples with different microbiological identification methods: An in vitro study
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number74
dspace.entity.typePublication
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