Measuring local-directional resolution and local anisotropy in cryo-EM maps

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dc.contributor.authorVilas, José Luis
dc.contributor.authorTagare, Hemant D.
dc.contributor.authorVargas Balbuena, Javier
dc.contributor.authorCarazo, Jose María
dc.contributor.authorSorzano, Carlos Óscar S.
dc.date.accessioned2026-01-08T21:53:04Z
dc.date.available2026-01-08T21:53:04Z
dc.date.issued2020-01-02
dc.description© The Author(s) 2020. Supplementary information is available for this paper at https://doi.org/10.1038/s41467019-13742-w. R01GM114051. RGPIN-2018-04813 NC-253837
dc.description.abstractThe introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
dc.description.departmentDepto. de Óptica
dc.description.facultyFac. de Ciencias Físicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipNational Institutes of Health (US)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipGouvernement du Québec
dc.description.sponsorshipEuropean Comission
dc.description.statuspub
dc.identifier.citationVilas, J.L., Tagare, H.D., Vargas, J. et al. Measuring local-directional resolution and local anisotropy in cryo-EM maps. Nat Commun 11, 55 (2020). https://doi.org/10.1038/s41467-019-13742-w
dc.identifier.doi10.1038/s41467-019-13742-w
dc.identifier.essn2041-1723
dc.identifier.officialurlhttps://doi.org/10.1038/s41467-019-13742-w
dc.identifier.relatedurlhttps://www.nature.com/articles/s41467-019-13742-w
dc.identifier.urihttps://hdl.handle.net/20.500.14352/129693
dc.journal.titleNature Communications
dc.language.isoeng
dc.page.final55-7
dc.page.initial55-1
dc.publisherNature Research
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI//BIO2016-76400-R/ES/FLEX3D: ANALISIS EN ALTO RENDIMIENTO DE LA FLEXIBILIDAD ESTRUCTURAL TANTO POR CRIO MICROSCOPIA ELECTRONICA COMO POR CRIO MICROSCOPIA DE RAYOS X BLANDOS/
dc.relation.projectIDS2017/BMD-3817/TOMOXLIVER
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/824087/EU
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/810057/EU
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu535
dc.subject.cdu004.91
dc.subject.keywordNuclear instruments
dc.subject.ucmÓptica (Física)
dc.subject.unesco2209.90 Tratamiento Digital. Imágenes
dc.titleMeasuring local-directional resolution and local anisotropy in cryo-EM maps
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number11
dspace.entity.typePublication
relation.isAuthorOfPublication6ccb1e60-8b61-4b23-8a0a-09af30f7b795
relation.isAuthorOfPublication.latestForDiscovery6ccb1e60-8b61-4b23-8a0a-09af30f7b795

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