Valvular Aortic Stenosis: A Proteomic Insight

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dc.contributor.authorGil Dones, Félix
dc.contributor.authorMartin-Rojas, Tatiana
dc.contributor.authorLopez-Almodovar, Luis
dc.contributor.authorCuesta, Fernando de La
dc.contributor.authorDarde, Veronica
dc.contributor.authorÁlvarez Llamas, Gloria
dc.contributor.authorJuarez-Tosina, Rocio
dc.contributor.authorBarroso, Gemma
dc.contributor.authorVivanco, Fernando
dc.contributor.authorPadial, Luis
dc.contributor.authorBarderas, Maria
dc.date.accessioned2024-01-23T13:01:12Z
dc.date.available2024-01-23T13:01:12Z
dc.date.issued2010
dc.descriptionThis work was supported by grants from the Instituto de Salud Carlos III (FIS PI070537 and FIS P080970), Fondo de Investigación Sanitaria de Castilla la Mancha (FISCAM PI2008/08) and CAM (Biomarkers S2006/GEN-0247). G. Alvarez-Llamas is a recipient of a research contract from the “Juan de la Cierva” program (JCI-2006-3349) of the Spanish Ministry of Science and Innovation. F. de la Cuesta was supported by the FIS (FI06/00583) and Tatiana Martin-Rojas was supported by Instituto de Salud Carlos III (RD06/0014/1015–RECAVA).
dc.description.abstractCalcified aortic valve disease is a slowly progressive disorder that ranges from mild valve thickening with no obstruction of blood flow, known as aortic sclerosis, to severe calcification with impaired leaflet motion or aortic stenosis. In the present work we describe a rapid, reproducible and effective method to carry out proteomic analysis of stenotic human valves by conventional 2-DE and 2D-DIGE, minimizing the interference due to high calcium concentrations. Furthermore, the protocol permits the aortic stenosis proteome to be analysed, advancing our knowledge in this area. Until recently, aortic stenosis (AS) was considered a passive process secondary to calcium deposition in the aortic valves. However, it has recently been highlighted that the risk factors associated with the development of calcified AS in the elderly are similar to those of coronary artery disease. Furthermore, degenerative AS shares histological characteristics with atherosclerotic plaques, leading to the suggestion that calcified aortic valve disease is a chronic inflammatory process similar to atherosclerosis. Nevertheless, certain data does not fit with this theory making it necessary to further study this pathology. The aim of this study is to develop an effective protein extraction protocol for aortic stenosis valves such that proteomic analyses can be performed on these structures. In the present work we have defined a rapid, reproducible and effective method to extract proteins and that is compatible with 2-DE, 2D-DIGE and MS techniques. Defining the protein profile of this tissue is an important and challenging task that will help to understand the mechanisms of physiological/pathological processes in aortic stenosis valves.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipAortic stenosis
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.sponsorshipCastilla la Mancha
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.statuspub
dc.identifier.citationGil-Dones F, Martin-Rojas T, Lopez-Almodovar LF, et al. Valvular Aortic Stenosis: A Proteomic Insight. Clinical Medicine Insights: Cardiology. 2010;4. doi:10.4137/CMC.S3884
dc.identifier.doi10.4137/cmc.s3884
dc.identifier.essn1179-5468
dc.identifier.officialurlhttps://doi.org/10.4137/CMC.S3884
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94763
dc.journal.titleClinical Medicine Insights: Cardiology
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.publisherSAGE Publications
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordProteomics
dc.subject.keywordHuman aortic valves
dc.subject.ucmCiencias
dc.subject.unesco24 Ciencias de la Vida
dc.titleValvular Aortic Stenosis: A Proteomic Insight
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number4
dspace.entity.typePublication
relation.isAuthorOfPublication0827e638-921a-4475-9a48-b859587719c5
relation.isAuthorOfPublication16e9b128-f6de-4009-817c-53e91375baa3
relation.isAuthorOfPublication.latestForDiscovery0827e638-921a-4475-9a48-b859587719c5
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