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Phosphorylcholine-Based Contact Lenses for Sustained Release of Resveratrol: Design, Antioxidant and Antimicrobial Performances, and In Vivo Behavior

dc.contributor.authorVivero López, María
dc.contributor.authorPereira da Mota, Ana Filipa
dc.contributor.authorCarracedo Rodríguez, Juan Gonzalo
dc.contributor.authorHuete Toral, Fernando
dc.contributor.authorParga Martínez, Ana
dc.contributor.authorOtero Casal, Ana María
dc.contributor.authorConcheiro Nine, Ángel
dc.contributor.authorAlvarez Lorenzo, Carmen
dc.date.accessioned2023-06-22T12:51:20Z
dc.date.available2023-06-22T12:51:20Z
dc.date.issued2022-12-10
dc.description.abstractDesign of advanced contact lenses (CLs) demands materials that are safe and comfortable for the wearers and that preserve the normal eye microbiota, avoiding chronic inflammation and biofilm development. This work aimed to combine the natural antibiofouling phosphorylcholine and the antioxidant and prebiotic resveratrol as integral components of CLs that may have the additional performance of preventing oxidative-stress related eye diseases. Different from previous uses of 2-methacryloyloxyethyl phosphorylcholine (MPC) as coating, we explored the feasibility of adding MPC at high proportions as a comonomer of 2-hydroxyethyl methacrylate (HEMA)-based hydrogels while still allowing for the loading of the hydrophobic resveratrol. Homogeneous distribution of MPC along the hydrogel depth (confirmed by Raman spectroscopy) notably increased solvent uptake and the proportion of free water while it decreased Young’s modulus. Relevantly, MPC did not hinder the uptake of resveratrol by CLs (>10 mg/g), which indeed showed network/water partition coefficients of >100. Protocols for CLs sterilization and loading of resveratrol under aseptic conditions were implemented, and the effects of tear proteins on resveratrol release rate were investigated. CLs sustained resveratrol release for more than 24 h in vitro, and sorption of albumin onto the hydrogel, although attenuated by MPC, slowed down the release. The combination of MPC and resveratrol reduced P. aeruginosa and S. aureus growth as tested in a novel hydrogel disk-agar interface biofilm growth setup. The developed CLs showed excellent anti-inflammatory properties and biocompatibility in in ovo and rabbit tests and provided higher and more prolonged levels of resveratrol in tear fluid, which favored resveratrol biodistribution in anterior and posterior eye segments compared to eye drops. Correlations between the release profiles of resveratrol in vitro and in vivo were assessed. Relevantly, the CLs preserved the antioxidant properties of resveratrol during the entire 8 h of wearing. In sum, CLs prepared with high proportion in MPC may help address safety and comfort requirements while having drug releasing capabilities.
dc.description.departmentDepto. de Optometría y Visión
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipMCIN - Ministerio de Ciencia e Innovación de España
dc.description.sponsorshipXunta de Galicia (España)
dc.description.sponsorshipFondos FEDER - European Union’s Horizon 2020
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77567
dc.identifier.doi10.1021/acsami.2c18217
dc.identifier.issn1944-8244, 1944-8252 (Online)
dc.identifier.officialurlhttps://doi.org/10.1021/acsami.2c18217
dc.identifier.relatedurlhttps://pubs.acs.org/doi/10.1021/acsami.2c18217#
dc.identifier.urihttps://hdl.handle.net/20.500.14352/73258
dc.issue.number50
dc.journal.titleApplied materials and interfaces
dc.language.isoeng
dc.page.final55446
dc.page.initial55431
dc.publisherACS
dc.relation.projectIDPID 2020-113881RB-I00/AEI/10.13039/501100011033
dc.relation.projectIDED431C 2020/17
dc.relation.projectIDED481A-2019/120
dc.relation.projectID813440
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu617.7-089.243
dc.subject.cdu681.73
dc.subject.keywordMedicated contact lenses
dc.subject.keywordresveratrol
dc.subject.keywordfree water
dc.subject.keywordantioxidant
dc.subject.keywordantimicrobial
dc.subject.keywordin vitro−in vivo correlations
dc.subject.keywordbacteria
dc.subject.keywordHydrogels
dc.subject.keywordPhytochemistry
dc.subject.ucmÓptica y optometría
dc.subject.ucmLentes de contacto
dc.subject.ucmMateriales ópticos
dc.subject.unesco2209 Óptica
dc.subject.unesco3311.11 Instrumentos ópticos
dc.subject.unesco2209.14 Propiedad ópticas de los sólidos
dc.titlePhosphorylcholine-Based Contact Lenses for Sustained Release of Resveratrol: Design, Antioxidant and Antimicrobial Performances, and In Vivo Behavior
dc.typejournal article
dc.volume.number14
dspace.entity.typePublication
relation.isAuthorOfPublication97433353-d31b-4cf5-b2c0-47d2e6703fe5
relation.isAuthorOfPublication5d71aa96-3e30-40bc-a2f0-4a12ff075bdb
relation.isAuthorOfPublication.latestForDiscovery97433353-d31b-4cf5-b2c0-47d2e6703fe5

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