Methanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells

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dc.contributor.authorReis, Filipa S.
dc.contributor.authorLima, Raquel T.
dc.contributor.authorMorales Gómez, Patricia
dc.contributor.authorFerreira, Isabel C. F. R.
dc.contributor.authorVasconcelos, M. Helena
dc.date.accessioned2023-06-18T06:06:46Z
dc.date.available2023-06-18T06:06:46Z
dc.date.issued2015-09-29
dc.description.abstractGanoderma lucidum is one of the most widely studied mushroom species, particularly in what concerns its medicinal properties. Previous studies (including those from some of us) have shown some evidence that the methanolic extract of G. lucidum affects cellular autophagy. However, it was not known if it induces autophagy or decreases the autophagic flux. The treatment of a gastric adenocarcinoma cell line (AGS) with the mushroom extract increased the formation of autophagosomes (vacuoles typical from autophagy). Moreover, the cellular levels of LC3-II were also increased, and the cellular levels of p62 decreased, confirming that the extract affects cellular autophagy. Treating the cells with the extract together with lysossomal protease inhibitors, the cellular levels of LC3-II and p62 increased. The results obtained proved that, in AGS cells, the methanolic extract of G. lucidum causes an induction of autophagy, rather than a reduction in the autophagic flux. To our knowledge, this is the first study proving that statement.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipe Portuguese Foundation for Science and Technology.
dc.description.sponsorshipPrograma Operacional Regional do Norte
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/69036
dc.identifier.doi10.3390/molecules201017872
dc.identifier.issn1420-3049
dc.identifier.officialurlhttps://doi.org/10.3390/molecules201017872
dc.identifier.relatedurlhttps://www.mdpi.com/1420-3049/20/10/17872
dc.identifier.urihttps://hdl.handle.net/20.500.14352/23899
dc.issue.number10
dc.journal.titleMolecules
dc.language.isoeng
dc.page.final17882
dc.page.initial17872
dc.publisherMDPI
dc.relation.projectIDPEst-C/SAU/LA0003/2013
dc.relation.projectIDNORTE-07-0162-FEDER00018-Contributos para o reforço da capacidade do IPATIMUP enquanto actor do sistema regional de inovação
dc.relation.projectIDNORTE-07-0162-FEDER-000067-Reforço e consolidação da capacidade infraestrutural do IPATIMUP para o sistema regional de inovação
dc.relation.projectIDPEst-OE/AGR/UI0690/2014
dc.relation.projectIDSFRH/BPD/68787/2010
dc.relation.projectIDNORTE-07-0124-FEDER-000023
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu616.3
dc.subject.cdu615.322
dc.subject.cdu615.27
dc.subject.keywordGanoderma lucidum
dc.subject.keywordmethanolic extract
dc.subject.keywordantitumor potential
dc.subject.keywordprogrammed cell death (PCD)
dc.subject.keywordautophagy
dc.subject.ucmDietética y nutrición (Farmacia)
dc.subject.ucmMicrobiología (Farmacia)
dc.subject.unesco3302.03 Microbiología Industrial
dc.titleMethanolic Extract of Ganoderma lucidum Induces Autophagy of AGS Human Gastric Tumor Cells
dc.typejournal article
dc.volume.number20
dspace.entity.typePublication
relation.isAuthorOfPublicationa28b2a5d-9fb3-497a-8758-6d2c73bce854
relation.isAuthorOfPublication.latestForDiscoverya28b2a5d-9fb3-497a-8758-6d2c73bce854
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