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Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor

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dc.contributor.authorFiorini, Emma
dc.contributor.authorReche, Pedro A
dc.date.accessioned2023-06-20T17:26:43Z
dc.date.available2023-06-20T17:26:43Z
dc.date.issued2002
dc.description.abstractNegative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamma irradiation-stimulated thymocyte death. The predicted protein contains seven ankyrin repeats and is homologous to I kappa B family members. In class I and class II MHC-restricted TCR transgenic mice, transcription of I kappa BNS is stimulated by peptides that trigger negative selection but not by those inducing positive selection (i.e., survival) or nonselecting peptides. I kappa BNS blocks transcription from NF-kappa B reporters, alters NF-kappa B electrophoretic mobility shifts, and interacts with NF-kappa B proteins in thymic nuclear lysates following TCR stimulation. Retroviral transduction of I kappa BNS in fetal thymic organ culture enhances TCR-triggered cell death consistent with its function in selection.
dc.description.departmentDepto. de Inmunología, Oftalmología y ORL
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/9342
dc.identifier.urihttps://hdl.handle.net/20.500.14352/58249
dc.language.isoeng
dc.rights.accessRightsopen access
dc.subject.ucmInmunología
dc.subject.ucmBiología molecular (Biología)
dc.subject.unesco2412 Inmunología
dc.subject.unesco2415 Biología Molecular
dc.titlePeptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor
dc.typejournal article
dspace.entity.typePublication
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