Publication: Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor
dc.contributor.author | Fiorini, Emma | |
dc.contributor.author | Reche, Pedro A | |
dc.date.accessioned | 2023-06-20T17:26:43Z | |
dc.date.available | 2023-06-20T17:26:43Z | |
dc.date.issued | 2002 | |
dc.description.abstract | Negative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamma irradiation-stimulated thymocyte death. The predicted protein contains seven ankyrin repeats and is homologous to I kappa B family members. In class I and class II MHC-restricted TCR transgenic mice, transcription of I kappa BNS is stimulated by peptides that trigger negative selection but not by those inducing positive selection (i.e., survival) or nonselecting peptides. I kappa BNS blocks transcription from NF-kappa B reporters, alters NF-kappa B electrophoretic mobility shifts, and interacts with NF-kappa B proteins in thymic nuclear lysates following TCR stimulation. Retroviral transduction of I kappa BNS in fetal thymic organ culture enhances TCR-triggered cell death consistent with its function in selection. | |
dc.description.department | Depto. de Inmunología, Oftalmología y ORL | |
dc.description.faculty | Fac. de Medicina | |
dc.description.refereed | TRUE | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/9342 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/58249 | |
dc.language.iso | eng | |
dc.rights.accessRights | open access | |
dc.subject.ucm | Inmunología | |
dc.subject.ucm | Biología molecular (Biología) | |
dc.subject.unesco | 2412 Inmunología | |
dc.subject.unesco | 2415 Biología Molecular | |
dc.title | Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor | |
dc.type | journal article | |
dspace.entity.type | Publication |
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