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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus

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dc.contributor.authorCastellanos, Elena
dc.contributor.authorAranaz Martín, Alicia
dc.contributor.authorDe Buck, Jeroen
dc.date.accessioned2023-06-20T04:20:43Z
dc.date.available2023-06-20T04:20:43Z
dc.date.issued2010-04
dc.description.abstractHigh-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39691
dc.identifier.doi10.1128/JCM.02484-09
dc.identifier.issn1098-660X
dc.identifier.officialurlhttp://jcm.asm.org/content/48/4/1474.full
dc.identifier.urihttps://hdl.handle.net/20.500.14352/45190
dc.issue.number4
dc.journal.titleJournal of clinical microbiology
dc.language.isoeng
dc.page.final7
dc.page.initial1474
dc.publisherAmerican Society for Microbiology
dc.relation.projectID(AP2005-0696 GAN)
dc.rights.accessRightsrestricted access
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleRapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus
dc.typejournal article
dc.volume.number48
dspace.entity.typePublication
relation.isAuthorOfPublication4243d2aa-49ad-4afc-8924-8fbf5d0feae7
relation.isAuthorOfPublication.latestForDiscovery4243d2aa-49ad-4afc-8924-8fbf5d0feae7

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