Bisphenol A in artificial soil: Effects on growth, reproduction and immunity in earthworms

Abstract

The application of biosolids in agricultural fields is increasing annually. They contain not only nutrients but also xenobiotics, such as Bisphenol A (BPA). These compounds are not regulated in the use of biosolids in agriculture, which highlights the need to assess their effects on soil life, of which earthworms are most abundant of the animal representatives. In this study the effect of BPA on life-history parameters, such as mortality, growth and reproduction, and on immunity, is evaluated for Dendrobaena veneta and Eisenia fetida. Sublethal concentrations were evaluated by a modified OECD artificial soil test. Decline in growth with increasing concentration of BPA was detected during the first two weeks and the opposite effect for the next two, although these differences were only significant at the highest concentration. Reproduction traits were only significantly different for E. fetida, for which the number of juveniles decreased at higher concentrations, thus showing different sensitivity in both species. By using a contact test, the potentially harmful effect of direct contact with BPA was shown to be much higher than in soil (resembling natural) conditions. Finally, results indicate that BPA may not affect the immune system of these animals, at least in terms of coelomocyte viability.