The association of Lactococcus petauri with lactococcosis is older than expected

Simple item page
dc.contributor.authorVela Alonso, Ana Isabel
dc.contributor.authorBlanco Gutiérrez, María Del Mar
dc.contributor.authorColussi, Silvia
dc.contributor.authorCharalampos, Kotzamanidis
dc.contributor.authorPrearo, Marino
dc.contributor.authorAltinok, Ilhan
dc.contributor.authorLuigi Acutis, Pier
dc.contributor.authorVolpatti, Donatella
dc.contributor.authorAlba, Patricia
dc.contributor.authorFeltrin, Fabiola
dc.contributor.authorIanzano, Angela
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.date.accessioned2024-05-24T14:02:52Z
dc.date.available2024-05-24T14:02:52Z
dc.date.issued2023-09-12
dc.description2023 Acuerdos transformativos CRUE
dc.description.abstractLactococcosis is a globally prevalent infectious disease that has a significant economic and sanitary impact on the rainbow trout industry. Lactococcus garvieae has traditionally been considered the only species implicated in the etiology of this disease, but Lactococcus petauri, a new species, has recently been implicated as another etiological agent. Both species cannot be distinguished by routine methods commonly used in diagnostic laboratories, resulting in their misidentification. In the present study, the identification of 48 isolates initially identified as L. garvieae was studied by determining their in-silico DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values using pairwise comparisons of their whole genome sequences and the genomes of the type strains of L. garvieae and L. petauri. The genome sequences of 37 isolates from countries in which lactococcosis can be considered endemic (Spain, Italy, Türkiye, and Greece) were obtained in this study, and the genomes of 11 isolates were retrieved from the GenBank database. Isolates from Italy, Singapore, Japan, South Korea, India, one Turkish isolate from 2013 and two Spanish isolates recovered in 1992 and 1996 were confirmed as L. garvieae. The remaining isolates from Spain and Türkiye, as well as those from Portugal, Israel, USA, and Greece were identified as L. petauri. Overall, 60.4% of isolates previously identified as L. garvieae were found to be L. petauri. These results confirm the implication of both species in the etiology of lactococcosis and suggest that L. petauri plays a significant role in the epidemiology of this disease. Some of the isolates identified as L. petauri in the present study were isolated three decades ago, indicating that its association with lactococcosis is older than might be expected from the recent descriptions. The commercial Rapid ID32 Strep system was unable to discriminate between L. garvieae and L. petauri. However, both species exhibited some biochemical differences that might serve as phenotypic markers for their presumptive recognition. Consequently, isolates that hydrolyze hippurate and produce acid from sucrose and tagatose could be presumptively recognized as L. petauri, while those that fail these tests could be identified as L. garvieae. The results of this work indicate that great attention should be given to L. petauri in the epidemiology of lactococcosis.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationVela, A. I., Del Mar Blanco, M., Colussi, S., Kotzamanidis, C., Prearo, M., Altinok, I., Acutis, P. L., Volpatti, D., Alba, P., Feltrin, F., Ianzano, A., Domínguez, L., & Fernández-Garayzábal, J. F. (2024). The association of Lactococcus petauri with lactococcosis is older than expected. Aquaculture, 578, 740057. https://doi.org/10.1016/j.aquaculture.2023.740057
dc.identifier.doi10.1016/j.aquaculture.2023.740057
dc.identifier.essn1873-5622
dc.identifier.issn0044-8486
dc.identifier.officialurlhttps://doi.org/10.1016/j.aquaculture.2023.740057
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104409
dc.issue.number740057
dc.journal.titleAquaculture
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.09
dc.subject.keywordLactococcosis
dc.subject.keywordLactococcus petauri
dc.subject.keywordLactococcus garvieae
dc.subject.keywordIdentification
dc.subject.keywordGenome sequences
dc.subject.keywordPhenotypic markers
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleThe association of Lactococcus petauri with lactococcosis is older than expected
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number578
dspace.entity.typePublication
relation.isAuthorOfPublication83481d48-83ea-472f-8b74-067bc00debee
relation.isAuthorOfPublicationfbae33df-e75a-4740-8236-9fc8cc45a322
relation.isAuthorOfPublication5d5c3513-e6d9-451a-ba7d-ba0634c84bcf
relation.isAuthorOfPublicationbaae9555-7eff-4de2-88b8-ad1c8bf8dd98
relation.isAuthorOfPublication.latestForDiscovery83481d48-83ea-472f-8b74-067bc00debee
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Association of Lactococus petauri.pdf
Size:
1.82 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos