Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2

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dc.contributor.authorRebollada Merino, Agustín Miguel
dc.contributor.authorGarcía-Seco Romero, María Teresa
dc.contributor.authorPérez Sancho, Marta
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorRodríguez Bertos, Antonio Manuel
dc.date.accessioned2024-08-20T11:17:37Z
dc.date.available2024-08-20T11:17:37Z
dc.date.issued2023-03-29
dc.description.abstractPorcine brucellosis, which is caused by Brucella suis biovar (bv) 2, is a re-emerging disease that causes reproductive problems in pigs in Europe. The pathogenesis and lesions of B. suis intrauterine infection are poorly characterized; characterization could facilitate the diagnosis and investigation of porcine brucellosis. We collected samples of placentas and fetuses for histologic and microbiologic studies during an outbreak of abortions on a pig-breeding farm in Spain. Brucella was cultured from the vaginal swabs obtained from sows that had aborted, some placentas, and fetal tissues (spleen, liver, lung, gastric content); molecular testing confirmed B. suis bv 2 infection. Histologically, there was necrotizing and hemorrhagic placentitis; suppurative hepatitis; lymphoid depletion and sinusoidal histiocytosis in the spleen, lymph nodes, and thymus; and bronchointerstitial pneumonia. Hemorrhages were observed in the umbilical cord, heart, kidneys, and brain. We detected Brucella by immunohistochemistry (IHC) in all of the placentas and fetal organs studied, specifically in the trophoblasts of the chorionic epithelium, in the cytoplasm of macrophages in the chorionic stroma, and extracellularly in necrotic debris. Furthermore, we assessed the lymphocyte population in the placentas through the use of IHC (anti-CD3, anti- Pax5 antibodies), revealing that the lymphocytic response was composed of T cells but not B cells.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades
dc.description.statuspub
dc.identifier.citationRebollada-Merino, A., García-Seco, T., Pérez-Sancho, M., Domínguez, L., & Rodríguez-Bertos, A. (2023). Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 35(3), 258–265. https://doi.org/10.1177/10406387231163867
dc.identifier.doi10.1177/10406387231163867
dc.identifier.issn1040-6387
dc.identifier.issn1943-4936
dc.identifier.officialurl10.1177/10406387231163867
dc.identifier.pmid36988301
dc.identifier.relatedurlhttps://journals.sagepub.com/doi/epub/10.1177/10406387231163867
dc.identifier.urihttps://hdl.handle.net/20.500.14352/107532
dc.issue.number3
dc.journal.titleJournal of Veterinary Diagnostic Investigation
dc.language.isoeng
dc.page.final265
dc.page.initial258
dc.publisherSage
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098658-B-C22/ES/EL SISTEMA DE DOS COMPONENTES WZM%2FWZT DE BRUCELLA: ESTUDIOS MOLECULARES, INTERACCIONES PATOGENO-HOSPEDADOR EN OVINO Y APLICACIONES EN B. SUIS/
dc.relation.projectIDRTI2018-098658-B-C22;
dc.relation.projectIDPRE2019-087439
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordBrucella suis biovar 2
dc.subject.keyworddiagnosis
dc.subject.keywordfetus
dc.subject.keywordhistopathology
dc.subject.keywordimmunohistochemistry
dc.subject.keywordplacenta
dc.subject.keywordswine
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleHistopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number35
dspace.entity.typePublication
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Histopathologic and immunohistochemical findings in the placentas and fetuses of domestic swine naturally infected with Brucella suis biovar 2
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