Global transcriptome analysis of Lactococcus garvieae strains in response to temperature

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dc.contributor.authorAguado Urda, Mónica
dc.contributor.authorGibello Prieto, Alicia
dc.contributor.authorBlanco Gutiérrez, María Del Mar
dc.contributor.authorLópez Alonso, Victoria
dc.contributor.authorLópez Campos, Guillermo H
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.date.accessioned2023-06-19T15:04:44Z
dc.date.available2023-06-19T15:04:44Z
dc.date.issued2013
dc.description.abstractLactococcus garvieae is an important fish and an opportunistic human pathogen. The genomic sequences of several L. garvieae strains have been recently published, opening the possibility of global studies on the biology of this pathogen. In this study, a whole genome DNA microarray of two strains of L. garvieae was designed and validated. This DNA microarray was used to investigate the effects of growth temperature (18°C and 37°C) on the transcriptome of two clinical strains of L. garvieae that were isolated from fish (Lg8831) and from a human case of septicemia (Lg21881). The transcriptome profiles evidenced a strain-specific response to temperature, which was more evident at 18°C. Among the most significant findings, Lg8831 was found to up-regulate at 18°C several genes encoding different cold-shock and cold-induced proteins involved in an efficient adaptive response of this strain to low-temperature conditions. Another relevant result was the description, for the first time, of respiratory metabolism in L. garvieae, whose gene expression regulation was temperature-dependent in Lg21881. This study provides new insights about how environmental factors such as temperature can affect L. garvieae gene expression. These data could improve our understanding of the regulatory networks and adaptive biology of this important pathogen.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39642
dc.identifier.doi10.1371/journal.pone.0079692
dc.identifier.issn1932-6203
dc.identifier.officialurlhttp://dx.doi.org/10.1371/journal.pone.0079692
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35283
dc.issue.number11
dc.journal.titlePloS one
dc.language.isoeng
dc.page.initiale79692
dc.publisherPublic Library Science
dc.relation.projectIDAGL2009-12447
dc.relation.projectIDAGL2012-35419
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleGlobal transcriptome analysis of Lactococcus garvieae strains in response to temperature
dc.typejournal article
dc.volume.number8
dspace.entity.typePublication
relation.isAuthorOfPublicationa3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85
relation.isAuthorOfPublicationfbae33df-e75a-4740-8236-9fc8cc45a322
relation.isAuthorOfPublicationbaae9555-7eff-4de2-88b8-ad1c8bf8dd98
relation.isAuthorOfPublication.latestForDiscoverya3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85
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