Soil Microbial Response to Cover Crop Termination Methods under Two Water Levels

Abstract

Cover crops (CC) promote soil health, but the termination method can condition the benefits for soil microorganisms. In a greenhouse experiment, we evaluated the legacy effects of four common CC termination methods on mycorrhization, soil microbial abundance, structure, and activity, as well as other soil properties, and its interaction with water levels (well-watered and water deficit). Mowing and residue incorporation (INC), glyphosate (GLY), roller crimper (ROL) and glyphosate + roller crimper (RGL) were evaluated, together with no CC, at two sampling dates of a subsequent maize. The water level modulated the soil microbial response to CC termination methods, especially in the glyphosate methods. Legacy effects on soil microbial attributes were notable and evolved differently from maize, from pre-emergence to ~3 months later. At final sampling, INC showed the best microbial response at both water levels, enhancing most microbial attributes. ROL was the second most beneficial method, especially in well-watered soil, promoting fungi but nullifying the CC positive effect on bacteria. Regardless of water level, GLY and RGL showed a similar microbial response. In well-watered soil, GLY and RGL had a negative effect on the total fungi, which separated the RGL response from the ROL. Overall, the time since CC termination and water level modulated the soil microbial response to the termination methods. Further research is needed to investigate CC termination impacts under different environmental conditions, in order to better understand the processes involved and provide farm-level recommendations.