Viologen-functionalized single-walled carbon nanotubes as carrier nanotags for electrochemical immunosensing. Application to TGF-β1 cytokine

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dc.contributor.authorSánchez Tirado, Esther
dc.contributor.authorArellano, Luis M.
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorLanga, Fernando
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-17T22:11:13Z
dc.date.available2023-06-17T22:11:13Z
dc.date.issued2017-12
dc.description.abstractViologen-SWCNT hybrids are synthesized by aryl-diazonium chemistry in the presence of isoamyl nitrite followed by condensation reaction of the resulting HOOC-PheSWCNT with 1-(3-aminoethyl)-4,4’-bipyridinium bromine and N-alkylation with 2- bromoethylamine. The V-Phe-SWCNT hybrids were characterized by using different spectroscopic techniques (FT-IR, Raman, UV-vis), TGA and Kaiser test. ViologenSWCNTs were used for the preparation of an electrochemical immunosensor for the determination of the transforming growth factor β1 (TGF-β1) cytokine considered as a reliable biomarker in several human diseases. The methodology involved preparation of VPhe-SWCNT(-HRP)-anti-TGF conjugates by covalent linkage of HRP and anti-TGF onto V-Phe-SWCNT hybrids. Biotinylated anti-TGF antibodies were immobilized onto 4- carboxyphenyl-functionalized SPCEs modified with streptavidin and a sandwich type 2 immunoassay was implemented for TGF-β1 with signal amplification using V-PheSWCNT(-HRP)-anti-TGF conjugates as carrier tags. The analytical characteristics exhibited by the as prepared immunosensor (range of linearity between 2.5 and 1000 pg mL-1 TGF-β1; detection limit of 0.95 pg mL-1 ) improve notably those reported with other previous immunosensors or ELISA kits. A great selectivity against other proteins was also found. The prepared immunosensor was validated by determining TGF-β1 in real saliva samples. Minimal sample treatment was required and the obtained results were in excellent agreement with those obtained by using a commercial ELISA kit.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipJunta de Comunidades de Castilla La Mancha
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/45120
dc.identifier.doi10.1016/j.bios.2017.06.063
dc.identifier.issn0956-5663(Print) , 1873-4235 (Online)
dc.identifier.officialurlhttp://www.sciencedirect.com/science/article/pii/S0956566317304487?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18188
dc.journal.titleBiosensors and Biolelectrics
dc.language.isoeng
dc.page.final247
dc.page.initial240
dc.publisherElsevier
dc.relation.projectIDCTQ2015-70023-R
dc.relation.projectIDCTQ2016- 79189-R
dc.relation.projectIDNANOAVANSENS (S2013/MT-3029)
dc.relation.projectIDPEII-2014-014-P
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordViologen
dc.subject.keywordTransforming growth factor β1
dc.subject.keywordCytokine Saliva Electrochemical immunosensor
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleViologen-functionalized single-walled carbon nanotubes as carrier nanotags for electrochemical immunosensing. Application to TGF-β1 cytokine
dc.typejournal article
dc.volume.number98
dspace.entity.typePublication
relation.isAuthorOfPublication8ce0e95b-049c-4147-be76-110e258443a1
relation.isAuthorOfPublication8a730734-cdf5-4b59-b017-12e2320c872e
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery8ce0e95b-049c-4147-be76-110e258443a1
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