Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

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dc.contributor.authorPérez Sancho, Marta
dc.contributor.authorVela Alonso, Ana Isabel
dc.contributor.authorHorcajo Iglesias, María Del Pilar
dc.contributor.authorUgarte Ruiz, María
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.contributor.authorFuente López, Ricardo De La
dc.date.accessioned2024-01-30T15:40:29Z
dc.date.available2024-01-30T15:40:29Z
dc.date.issued2018-10-03
dc.description.abstractStaphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius and 22 subsp. aureus) recovered from different origins, countries, and years. The on-board library identification system correctly identified 42 of 52 (81%) S. aureus isolates at the species level with score values >2.0. Limited performance was observed for differentiation of S. aureus subspecies (particularly subsp. anaerobius). Visual inspection of MALDI-TOF MS profiles identified 5 subspecies-specific mass peaks ( m/ z 3430 and 6861 in S. aureus subsp. anaerobius, and m/ z 4046, 6890, and 8093 in S. aureus subsp. aureus) with 100% sensitivity and specificity values, which is potentially useful for differentiating these subspecies. The suitability of 3 models, Genetic Analysis (GA), Quick Classifier (QC), and Supervised Neural Network, for automatic identification of both subspecies was evaluated using the Recognition Capability (RC) and Cross Validation (CV) values provided by the on-board ClinProTools software. The GA and QC models reached RC and CV values of 100%. Both models were externally validated using a panel of 26 S. aureus isolates of both subspecies, with both models correctly classifying all isolates of both subspecies. MALDI-TOF MS coupled with ClinProTools software represents a rapid and simple approach for S. aureus subspecies discrimination.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipRegional Government of Madrid structural funds and European Union
dc.description.statuspub
dc.identifier.citationPérez-Sancho M, Vela AI, Horcajo P, Ugarte-Ruiz M, Domínguez L, Fernández-Garayzábal JF, de la Fuente R. Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles. J Vet Diagn Invest. 2018 Nov;30(6):813-820. doi: 10.1177/1040638718805537. Epub 2018 Oct 3. PMID: 30280650; PMCID: PMC6505833.
dc.identifier.doi10.1177/1040638718805537
dc.identifier.issn10406387
dc.identifier.officialurlhttps://journals.sagepub.com/doi/10.1177/1040638718805537
dc.identifier.pmid30280650
dc.identifier.relatedurlhttps://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6505833&blobtype=pdf
dc.identifier.relatedurlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505833/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96654
dc.issue.number6
dc.language.isoeng
dc.page.total8
dc.publisherSAGE publications
dc.relation.ispartofseriesJournal of Veterinary Diagnostic Investigation
dc.relation.projectIDS2013/ABI-2747 (TAVS-CM)
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordStaphylococcus aureus
dc.subject.keywordMALDI-TOF MS
dc.subject.keyworddifferentiation
dc.subject.keywordsubspecies
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleRapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles
dc.typeworking paper
dc.type.hasVersionAM
dc.volume.number30
dspace.entity.typePublication
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