Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality

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dc.contributor.authorCajas Suárez, Yulia Nathaly
dc.contributor.authorCañón Beltrán, Karina Esperanza
dc.contributor.authorMazzarella, Rosane
dc.contributor.authorNúñez Puente, Carolina
dc.contributor.authorGonzález Martínez, María Encina
dc.contributor.authorRodríguez Martínez, Heriberto
dc.contributor.authorRizos, Dimitri
dc.contributor.authorMartínez Serrano, Cristina A.
dc.date.accessioned2024-05-06T13:18:27Z
dc.date.available2024-05-06T13:18:27Z
dc.date.issued2024-04-22
dc.descriptionAuthorship contribution statement: Yulia N. Cajas: Writing – review & editing, Writing – original draft, Validation, Methodology, Investigation, Formal analysis, Conceptualization. Karina Canon-Beltran: Writing – review & editing, Writing – original draft, Validation, Methodology, Formal analysis, Conceptualization. Rosane Mazzarella: Writing – review & editing, Methodology. Carolina Nunez-Puente: Writing – review & editing, Methodology. Encina M. Gonzalez: Writing – review & editing, Conceptualization. Heriberto Rodriguez-Martinez: Writing – review & editing, Funding acquisition, Conceptualization. Dimitrios Rizos: Writing – review & editing, Supervision, Project administration, Funding acquisition, Conceptualization. Cristina A. Martinez-Serrano: Writing – review & editing, Writing – original draft, Validation, Methodology, Investigation, Formal analysis, Conceptualization.
dc.description.abstractIn vitro embryo production (IVP) is of great importance to the porcine industry, as well as for basic research and biomedical applications. Despite the large efforts made in laboratories worldwide to address suboptimal culture conditions, porcine IVP remains inefficient. Nobiletin (Nob, 5,6,7,8,3′,4′ hexamethoxyflavone) supplementation to in vitro culture (IVC) medium, enhances in vitro embryo development in various species. However, its impact on the quality and developmental capacity of in vitro-produced pig embryos is yet to be established. This study evaluated the effects of different concentrations (2.5 and 5 μM) of Nob during the early culture of in vitro-produced pig embryos on embryo developmental competence, mitochondrial activity, lipid content, intracellular Reactive Oxygen Species (ROS) and Glutathione (GSH) content, Total Cell Number (TCN) per blastocyst, and expression of genes related to embryo development, quality and oxidative stress. Embryos cultured in medium without Nob supplementation and in medium supplemented with 0.01 % dimethyl sulfoxide (DMSO-vehicle for Nob) constituted the Control and DMSO groups, respectively. Embryo development rates were evaluated on Days 2, 6 and 7 of IVC. Additionally, a representative group of embryos was selected to assess mitochondrial activity, lipid, ROS and GSH content (on Days 2 and 6 of IVC), TCN assessment and gene expression analyses (on Day 6 of IVC). No significant differences were observed in any of the parameters evaluated on Day 2 of IVC. In contrast, embryos cultured under the presence of Nob 2.5 showed higher developmental rates on Days 6 and 7 of IVC. In addition, Day 6 embryos showed increased mitochondrial activity, with decreased levels of ROS and GSH in the Nob 2.5 group compared to the other groups. Both Nob 2.5 and Nob 5 embryos showed higher TCN compared to the Control and DMSO groups. Furthermore, Nob 2.5 and Nob 5 upregulated the expression of Superoxide dismutase type 1 (SOD1) and Glucose-6-phosphate dehydrogenase (G6PDH) genes, which could help to counteract oxidative stress during IVC. In conclusion, the addition of Nob during the first 48 h of IVC increased porcine embryo development rates and enhanced their quality, including the upregulation of relevant genes that potentially improved the overall efficiency of the IVP system.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Veterinaria)
dc.description.departmentSección Deptal. de Anatomía y Embriología (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.statuspub
dc.identifier.essn1879-3231
dc.identifier.issn0093-691X
dc.identifier.officialurlhttps://doi.org/10.1016/j.theriogenology.2024.04.011
dc.identifier.pmid38669840
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103743
dc.journal.titleTheriogenology
dc.language.isoeng
dc.page.final46
dc.page.initial36
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MCIN/AEI/10.13039/501100011033
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordAntioxidant
dc.subject.keywordEmbryo
dc.subject.keywordIn vitro
dc.subject.keywordNobiletin
dc.subject.keywordPig
dc.subject.keywordROS
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleNobiletin as a novel agent to enhance porcine in vitro embryo development and quality
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number223
dspace.entity.typePublication
relation.isAuthorOfPublicationf4da9b8e-f83c-49ec-adff-fafcd0bf3ef7
relation.isAuthorOfPublication252147fa-6c76-4c5d-b884-af3fa2c738c3
relation.isAuthorOfPublication4aad6e28-b11f-4751-bfd4-3c419f05dc19
relation.isAuthorOfPublication.latestForDiscoveryf4da9b8e-f83c-49ec-adff-fafcd0bf3ef7
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