First bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin

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dc.contributor.authorMuñoz San Martín, Cristina
dc.contributor.authorMontero Calle, Ana
dc.contributor.authorGarranzo Asensio, María
dc.contributor.authorGamella Carballo, Maria
dc.contributor.authorPérez Ginés, Víctor
dc.contributor.authorPedrero Muñoz, María
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorBarderas, Rodrigo
dc.contributor.authorSantos Álvarez, Noemí de los
dc.contributor.authorLobo Castañón, María Jesús
dc.contributor.authorCampuzano Ruiz, Susana
dc.date.accessioned2023-06-22T12:30:01Z
dc.date.available2023-06-22T12:30:01Z
dc.date.issued2022-11-08
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2022)
dc.description.abstractThe glycosylation status of proteins is increasingly used as biomarker to improve the reliability in the diagnosis and prognosis of diseases as relevant as cancer. This feeds the need for tools that allow its simple and reliable analysis and are compatible with applicability in the clinic. With this objective in mind, this work reports the frst bioelectronic immunoplatforms described to date for the determination of glycosylated haptoglobin (Hp) and the simultaneous determination of total and glycosylated Hp. The bioelectronic immunoplatform is based on the implementation of non-competitive bioassays using two diferent antibodies or an antibody and a lectin on the surface of commercial magnetic microcarriers. The resulting bioconjugates are labeled with the horseradish peroxidase (HRP) enzyme, and after their magnetic capture on disposable electroplatforms, the amperometric transduction using the H2O2/hydroquinone (HQ) system allows the single or multiple detection. The developed immunoplatform achieves limits of detection (LODs) of 0.07 and 0.46 ng mL−1 for total and glycosylated Hp in bufer solution, respectively. The immunoplatform allows accurate determination using simple and relatively short protocols (approx. 75 min) of total and glycosylated Hp in the secretomes of in vitro–cultured colorectal cancer (CRC) cells with diferent metastatic potentials, which is not feasible, due to lack of sensitivity, by means of some commercial ELISA kits and Western blot methodology.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipInstituto de Salud Carlos III (ISCIII)/FEDER
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/75678
dc.identifier.doi10.1007/s00216-022-04397-6
dc.identifier.issn1618-2642
dc.identifier.officialurlhttps://doi.org/10.1007/s00216-022-04397-6
dc.identifier.urihttps://hdl.handle.net/20.500.14352/72692
dc.journal.titleAnalytical and Bioanalytical Chemistry
dc.language.isoeng
dc.publisherSpringer Nature
dc.relation.projectIDPID2019-103899RBI00 and RTI-2018-095756-B-I00
dc.relation.projectIDPI17CIII/00045 and PI20CIII/00019
dc.relation.projectIDTRANSNANOAVANSENS-CM (Grant S2018/NMT-4349)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordGlycosylated haptoglobin
dc.subject.keywordAmperometry
dc.subject.keywordMultiplexed immunoplatform
dc.subject.keywordSecretome
dc.subject.keywordMetastatic CRC cells
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleFirst bioelectronic immunoplatform for quantitative secretomic analysis of total and metastasis-driven glycosylated haptoglobin
dc.typejournal article
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery8ef8a354-3b10-4ce1-9125-3dcd418e5313
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