Antibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model

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dc.contributor.authorBueno, Jaime
dc.contributor.authorVirto Ruiz, Leire
dc.contributor.authorToledano Osorio, Manuel
dc.contributor.authorFiguero Ruiz, Elena
dc.contributor.authorToledano, Manuel
dc.contributor.authorMedina Castillo, Antonio L.
dc.contributor.authorOsorio, Raquel
dc.contributor.authorSanz Alonso, Mariano
dc.contributor.authorHerrera González, David
dc.date.accessioned2024-01-11T09:44:21Z
dc.date.available2024-01-11T09:44:21Z
dc.date.issued2022-01-18
dc.description.abstractThis investigation aimed to evaluate the antibacterial effect of polymeric nanoparticles (NPs), functionalized with calcium, zinc, or doxycycline, using a subgingival biofilm model of six bacterial species (Streptococcus oralis, Actinomyces naeslundii, Veillonela parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) on sandblasted, large grit, acid-etched titanium discs (TiDs). Undoped NPs (Un-NPs) or doped NPs with calcium (Ca-NPs), zinc (Zn-NPs), or doxycycline (Dox-NPs) were applied onto the TiD surfaces. Uncovered TiDs were used as negative controls. Discs were incubated under anaerobic conditions for 12, 24, 48, and 72 h. The obtained biofilm structure was studied by scanning electron microscopy (SEM) and its vitality and thickness by confocal laser scanning microscopy (CLSM). Quantitative polymerase chain reaction of samples was used to evaluate the bacterial load. Data were evaluated by analysis of variance (p < 0.05) and post hoc comparisons with Bonferroni adjustments (p < 0.01). As compared with uncovered TiDs, Dox-NPs induced higher biofilm mortality (47.21% and 85.87%, respectively) and reduced the bacterial load of the tested species, after 72 h. With SEM, scarce biofilm formation was observed in Dox-NPs TiDs. In summary, Dox-NPs on TiD reduced biofilm vitality, bacterial load, and altered biofilm formation dynamics.
dc.description.departmentDepto. de Anatomía y Embriología
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipMinistry of Economy and Competitiveness
dc.description.sponsorshipEuropean Regional Development Fund (FEDER)
dc.description.sponsorshipMinistry of Universities
dc.description.statuspub
dc.identifier.citationBueno, J.; Virto, L.; Toledano-Osorio, M.; Figuero, E.; Toledano, M.; Medina-Castillo, A.L.; Osorio, R.; Sanz, M.; Herrera, D. Antibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model. Polymers 2022, 14, 358. h
dc.identifier.doi10.3390/polym14030358
dc.identifier.essn2073-4360
dc.identifier.officialurlhttps://doi.org/10.3390/polym14030358
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92445
dc.issue.number3
dc.journal.titlePolymers
dc.language.isoeng
dc.publisherMPDI
dc.relation.projectIDPID2020-114694RB-I00 MINECO/AEI/FEDER/UE
dc.relation.projectIDFPU20/00450
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu616.314
dc.subject.keywordpolymers; nanoparticles; doxycycline; antibacterial; zinc; calcium; biofilm
dc.subject.ucmOdontología (Odontología)
dc.subject.unesco2302 Bioquímica
dc.titleAntibacterial Effect of Functionalized Polymeric Nanoparticles on Titanium Surfaces Using an In Vitro Subgingival Biofilm Model
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number14
dspace.entity.typePublication
relation.isAuthorOfPublication2bbceacb-841f-4572-81a1-d4ebc3aa9329
relation.isAuthorOfPublication780965c6-ea4d-4bf1-bbda-99914490ba50
relation.isAuthorOfPublication64cb103c-84c2-43c3-a4fc-60b1e598007d
relation.isAuthorOfPublication92a06fae-e947-48dc-b945-32283225e711
relation.isAuthorOfPublication.latestForDiscovery2bbceacb-841f-4572-81a1-d4ebc3aa9329
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