New insights into the genome of Rhodococcus ruber strain Chol-4

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dc.contributor.authorGuevara Acosta, Flor Govinda
dc.contributor.authorCastillo López, María
dc.contributor.authorAlonso, Sergio
dc.contributor.authorPerera González, Julián
dc.contributor.authorNavarro Llorens, Juana María
dc.date.accessioned2023-06-17T13:32:41Z
dc.date.available2023-06-17T13:32:41Z
dc.date.issued2019
dc.description.abstractBackground: Rhodococcus ruber strain Chol-4, a strain isolated from a sewage sludge sample, is able to grow in minimal medium supplemented with several compounds, showing a broad catabolic capacity. We have previously determined its genome sequence but a more comprehensive study of their metabolic capacities was necessary to fully unravel its potential for biotechnological applications. Results: In this work, the genome of R. ruber strain Chol-4 has been re-sequenced, revised, annotated and compared to other bacterial genomes in order to investigate the metabolic capabilities of this microorganism. The analysis of the data suggests that R. ruber Chol-4 contains several putative metabolic clusters of biotechnological interest, particularly those involved on steroid and aromatic compounds catabolism. To demonstrate some of its putative metabolic abilities, R. ruber has been cultured in minimal media containing compounds belonging to several of the predicted metabolic pathways. Moreover, mutants were built to test the naphtalen and protocatechuate predicted catabolic gene clusters. Conclusions: The genomic analysis and experimental data presented in this work confirm the metabolic potential of R. ruber strain Chol-4. This strain is an interesting model bacterium due to its biodegradation capabilities. The results obtained in this work will facilitate the application of this strain as a biotechnological tool.en
dc.description.abstractNuestro grupo de investigación de Ingeniería Metabólica de la UCM trabaja entre otros temas con la degradación bacteriana de aromáticos. Dentro de esta línea, se aisló y caracterizó a Rhodococcus ruber strain Chol-4 de aguas residuales por su capacidad de crecer en colesterol. En este artículo analizamos el genoma de esta cepa y estudiamos sus capacidades metabólicas como primer paso para su utilización como chasis biotecnológico.es
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/57478
dc.identifier.citationGuevara G, Castillo Lopez M, Alonso S, Perera J, Navarro-Llorens JM. New insights into the genome of Rhodococcus ruber strain Chol-4. BMC Genomics. 2019 May 2;20(1):332. doi: 10.1186/s12864-019-5677-2. PMID: 31046661; PMCID: PMC6498646.
dc.identifier.doi10.1186/s12864-019-5677-2
dc.identifier.issn1471-2164
dc.identifier.officialurlhttps//doi.org/10.1186/s12864-019-5677-2
dc.identifier.pmid31046661
dc.identifier.relatedurlhttps://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-019-5677-2
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13724
dc.issue.number332
dc.journal.titleBMC Genomics
dc.language.isoeng
dc.page.final17
dc.page.initial1
dc.page.total17
dc.publisherBMC
dc.relation.projectIDinfo:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación//RTC2014-2249-1
dc.relation.projectIDinfo:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación//BIO2012– 39695-CO2–01
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu575
dc.subject.cdu577.1
dc.subject.keywordRhodococcus ruber
dc.subject.keywordCatabolism
dc.subject.keywordBiodegradation
dc.subject.keywordGenome analysis
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmGenética
dc.subject.unesco2302 Bioquímica
dc.subject.unesco2409 Genética
dc.titleNew insights into the genome of Rhodococcus ruber strain Chol-4en
dc.title.alternativeNuevas perspectivas del genoma de Rhodooccus ruber strain Chol-4es
dc.typejournal article
dc.volume.number20
dspace.entity.typePublication
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relation.isAuthorOfPublication5a9a01b6-fb5f-422a-b3d0-74ad581e2cbd
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relation.isAuthorOfPublication.latestForDiscovery5a9a01b6-fb5f-422a-b3d0-74ad581e2cbd
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