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Evaluating aflatoxin gene expression in aspergillus section flavi

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dc.book.titleMycotoxigenic Fungi
dc.contributor.authorAzevedo Rodrigues, Paula Cristina
dc.contributor.authorGil Serna, Jessica
dc.contributor.authorGonzález Jaén, María Teresa
dc.date.accessioned2023-06-18T00:21:58Z
dc.date.available2023-06-18T00:21:58Z
dc.date.issued2017
dc.description.abstractThe determination of aflatoxin production ability and differentiation of aflatoxigenic strains can be assessed by monitoring the expression of one or several key genes using reverse transcription polymerase chain reaction (RT-PCR). We herein describe the methods for RNA induction, extraction, and quality determination, and the RT-PCR conditions used to evaluate the ability of a given Aspergillus strain to produce aflatoxins.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44036
dc.identifier.doi10.1007/978-1-4939-6707-0_15
dc.identifier.isbn978-1-4939-6705-6 (Print) 978-1-4939-6707-0 (Online)
dc.identifier.officialurlhttps://link.springer.com/book/10.1007%2F978-1-4939-6707-0
dc.identifier.urihttps://hdl.handle.net/20.500.14352/19455
dc.issue.number1542
dc.language.isoeng
dc.page.final247
dc.page.initial237
dc.publisherSpringer
dc.relation.ispartofseriesMethods in Molecular Biology: Methods and Protocols
dc.rights.accessRightsrestricted access
dc.subject.cdu582.282.123.4
dc.subject.cdu575
dc.subject.cdu615.372
dc.subject.keywordAspergillus flavus
dc.subject.keywordMycotoxins
dc.subject.keywordAflatoxigenic fungi
dc.subject.keywordRNA extraction
dc.subject.keywordRT-PCR
dc.subject.keywordGel electrophoresis
dc.subject.ucmBiología
dc.subject.ucmGenética
dc.subject.ucmMicrobiología (Biología)
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2409 Genética
dc.subject.unesco2414 Microbiología
dc.titleEvaluating aflatoxin gene expression in aspergillus section flavi
dc.typebook part
dspace.entity.typePublication
relation.isAuthorOfPublication413efe47-272c-4f1a-aae9-722b00567716
relation.isAuthorOfPublicationc6e49c5c-cd3d-4aa3-b582-4a5221825dc0
relation.isAuthorOfPublication.latestForDiscoveryc6e49c5c-cd3d-4aa3-b582-4a5221825dc0

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