Comparative evaluation of the performance of electrochemical immunosensors using magnetic microparticles and nanoparticles. Application to the determination of tyrosine kinase receptor AXL

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dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorBatlle, Montserrat
dc.contributor.authorGarcía de Frutos, Pablo
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-25T11:38:15Z
dc.date.available2024-01-25T11:38:15Z
dc.date.issued2017
dc.description.abstractElectrochemical sandwich immunoassay strategies involving the use of carboxyl-functionalized magnetic microbeads (cMBs) and magnetic nanoparticles (cMNPs) have been evaluated and compared. The proteolytically cleaved soluble Tyrosine kinase receptor sAXL was used as the target analyte. Antibodies against AXL were covalently immobilized on cMBs or cMNPs. Immunobinding of AXL was detected by means of a secondary biotinylated antibody and a streptavidinhorseradish peroxidase conjugate. The electrochemical transduction was accomplished by capturing the cMBs or cMNPs bearing the immunoconjugates onto screen-printed carbon electrodes (SPCEs) by using a small magnet. The amperometric response was measured at −0.20 V (vs the silver pseudoreference electrode of the SPCE) upon the addition of H2O2 in the presence of hydroquinone as the redox mediator. The calibration plots for AXL extended up to 7.5 ng mL−1 when cMBs were used for the preparation of the immunosensor and up to 40 ng mL−1 in the case of using cMNPs. The respective slope values were 158 (cMBs) and 43 nA mL ng−1 (cMNPs), while the achieved LODs were 74 (cMBs) and 75 pg mL−1 (cMNPs). Although the immunosensors prepared with cMBs provided a shorter range of linearity, they exhibited a 3.7-times larger sensitivity than those constructed with cMNPs. The successful application of the new strategies was demonstrated for the determination of the endogenous content of sAXL in real human serum samples (a cut-off value of 71 ng mL−1 have been established for patients with risk of heart failure). The immunosensors constructed using cMBs or cMNPs can be advanta geously compared, in terms of sensitivity and fabrication time, with the only immunosensor for AXL previously reported. In addition, these new immunosensors took approximately half time than ELISA to perform the assay.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationSerafín V, Torrente-Rodríguez R.M., Batlle M, P. García de Frutos P, Campuzano S, P. Yáñez-Sedeño P, Pingarrón J.M. Comparative evaluation of the performance of electrochemical immunosensors using magnetic microparticles and nanoparticles. application to the determination of tyrosine kinase receptor AXL. Electrochim. Acta. 2017 Agosto 24; 184: 4251-58
dc.identifier.doi10.1007/s00604-017-2455-1
dc.identifier.essn1436-5073
dc.identifier.issn0026-3672
dc.identifier.officialurlhttps://doi.org/10.1007/s00604-017-2455-1
dc.identifier.relatedurlhttps://produccioncientifica.ucm.es/investigadores/142605/colaboracion/investigador/4779643
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95386
dc.journal.titleMicrochimica Acta
dc.language.isoeng
dc.page.final4258
dc.page.initial4251
dc.publisherSpringer Wien
dc.relation.projectIDRTC-2015-4184-1
dc.relation.projectIDCTQ2015-70023-R
dc.relation.projectIDCTQ2015-64402-C2-1-R
dc.relation.projectIDS2013/ MT-3029
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu544.6
dc.subject.keywordElectrochemical immunosensor
dc.subject.keywordMagnetic microbeads
dc.subject.keywordMagnetic nanoparticles
dc.subject.keywordAXL
dc.subject.keywordHuman serum
dc.subject.keywordHeart failure
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleComparative evaluation of the performance of electrochemical immunosensors using magnetic microparticles and nanoparticles. Application to the determination of tyrosine kinase receptor AXL
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number184
dspace.entity.typePublication
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