Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells
dc.contributor.author | Carrión Herrero, Francisco Javier | |
dc.contributor.author | Domínguez Bernal, Gustavo Ramón | |
dc.contributor.author | Martínez Rodrigo, Abel | |
dc.contributor.author | Mas Zubiri, Alicia | |
dc.contributor.author | Blanco Gutiérrez, María Del Mar | |
dc.contributor.author | Orden Gutiérrez, José Antonio | |
dc.contributor.author | Fuente López, Ricardo De La | |
dc.date.accessioned | 2024-01-18T07:55:28Z | |
dc.date.available | 2024-01-18T07:55:28Z | |
dc.date.issued | 2017-10 | |
dc.description.abstract | Here, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection. | |
dc.description.department | Depto. de Sanidad Animal | |
dc.description.faculty | Fac. de Veterinaria | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Biodesign Institute | |
dc.description.sponsorship | Universidad Complutense de Madrid-Santander | |
dc.description.sponsorship | Arizona State University | |
dc.description.sponsorship | Comunidad de Madrid | |
dc.description.sponsorship | PLATESA (S2013/ABI-2906) | |
dc.description.sponsorship | Ministerio de Economía y Competitividad | |
dc.description.sponsorship | AGL2013-44100R, S2013/ABI-2906 | |
dc.description.status | pub | |
dc.identifier.citation | Domínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.002 | |
dc.identifier.doi | 10.1016/j.cimid.2017.07.002 | |
dc.identifier.essn | 0147-9571 | |
dc.identifier.officialurl | https://www.sciencedirect.com/science/article/pii/S0147957117300577 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/93736 | |
dc.issue.number | October 2017 | |
dc.journal.title | Comparative Immunology, Microbiology and Infectious Diseases | |
dc.language.iso | eng | |
dc.page.final | 19 | |
dc.page.initial | 13 | |
dc.publisher | Elsevier | |
dc.rights | Attribution 4.0 International | en |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.cdu | 636.09 | |
dc.subject.keyword | Dendritic cells | |
dc.subject.keyword | HisAK70 | |
dc.subject.keyword | Leishmaniosis | |
dc.subject.keyword | Salmonella | |
dc.subject.keyword | Vaccination | |
dc.subject.ucm | Veterinaria | |
dc.subject.unesco | 24 Ciencias de la Vida | |
dc.title | Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells | |
dc.type | journal article | |
dc.type.hasVersion | AM | |
dc.volume.number | 54 | |
dspace.entity.type | Publication | |
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relation.isAuthorOfPublication.latestForDiscovery | 8207c445-ff65-45fc-9070-60fe6e14b5e2 |
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