Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells

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dc.contributor.authorCarrión Herrero, Francisco Javier
dc.contributor.authorDomínguez Bernal, Gustavo Ramón
dc.contributor.authorMartínez Rodrigo, Abel
dc.contributor.authorMas Zubiri, Alicia
dc.contributor.authorBlanco Gutiérrez, María Del Mar
dc.contributor.authorOrden Gutiérrez, José Antonio
dc.contributor.authorFuente López, Ricardo De La
dc.date.accessioned2024-01-18T07:55:28Z
dc.date.available2024-01-18T07:55:28Z
dc.date.issued2017-10
dc.description.abstractHere, we describe a novel approach that exploits an attenuated mutant of Salmonella enterica serovar Choleraesuis as carrier to deliver a plasmid encoding protein HisAK70. Subsequently, dendritic cells (DCs) were pulsed with this vaccine vector. The aim of this study was to evaluate the effectiveness of the prepared HisAK70-S. Choleraesuis-pulsed DCs (HisAK70-SAL DCs) against visceral leishmaniosis (VL). In our ex vivo model of infection, the prepared formulations could decrease parasite growth by up to 80% by augmenting the production of IL-12p40 and by reducing arginase activity (ARG). Also, BALB/c mice when immunised with this formulation showed significant reduction in parasite burden in both spleen (20% of reduction) and liver (75% of reduction). The balance of the immune ratios IFN-γ/IL-10, TNF-α/IL-10, and IgG2a/IgG1 reflected the acquisition of an improved resistant phenotype in HisAK70-SAL DCs vaccinated mice compared to control mice. Our results suggest that HisAK70-SAL DCs could be a promising alternative approach for vaccine delivery that has the potential to fight Leishmania infantum (L. infantum) infection.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipBiodesign Institute
dc.description.sponsorshipUniversidad Complutense de Madrid-Santander
dc.description.sponsorshipArizona State University
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipPLATESA (S2013/ABI-2906)
dc.description.sponsorshipMinisterio de Economía y Competitividad
dc.description.sponsorshipAGL2013-44100R, S2013/ABI-2906
dc.description.statuspub
dc.identifier.citationDomínguez-Bernal, G., Martínez-Rodrigo, A., Mas, A., Blanco, M. M., Orden, J. A., De La Fuente, R., & Carrión, J. (2017). Alternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells. Comparative immunology, microbiology and infectious diseases, 54, 13–19. https://doi.org/10.1016/j.cimid.2017.07.002
dc.identifier.doi10.1016/j.cimid.2017.07.002
dc.identifier.essn0147-9571
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0147957117300577
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93736
dc.issue.numberOctober 2017
dc.journal.titleComparative Immunology, Microbiology and Infectious Diseases
dc.language.isoeng
dc.page.final19
dc.page.initial13
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordDendritic cells
dc.subject.keywordHisAK70
dc.subject.keywordLeishmaniosis
dc.subject.keywordSalmonella
dc.subject.keywordVaccination
dc.subject.ucmVeterinaria
dc.subject.unesco24 Ciencias de la Vida
dc.titleAlternative strategy for visceral leishmaniosis control: HisAK70-Salmonella Choleraesuis-pulsed dendritic cells
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number54
dspace.entity.typePublication
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