Characterization of Neospora caninum virulence factors NcGRA7 and NcROP40 in bovine target cells

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dc.contributor.authorRico San Román, Laura
dc.contributor.authorAmieva, Rafael
dc.contributor.authorHorcajo Iglesias, María Del Pilar
dc.contributor.authorGarcía Sánchez, Marta Eulalia
dc.contributor.authorPastor Fernández, Iván
dc.contributor.authorOrtega Mora, Luis Miguel
dc.contributor.authorCollantes Fernández, Esther
dc.date.accessioned2023-11-24T19:17:30Z
dc.date.available2023-11-24T19:17:30Z
dc.date.issued2023-06-14
dc.description.abstractBovine neosporosis is one of the major causes of reproductive failure in cattle worldwide, and differences in virulence between isolates have been widely shown. However, the molecular basis and mechanisms underlying virulence in Neospora caninum are mostly unknown. Recently, we demonstrated the involvement of NcGRA7 and NcROP40 in the virulence of N. caninum in a pregnant murine model using single knockout mutants in these genes generated by CRISR/Cas9 technology. In this study, the role of these proteins was investigated in two in vitro models using bovine target cells: trophoblast (F3 cell line) and monocyte-derived macrophages (BoMØ). The proliferation capacity of the single knockout mutant parasites was compared to the wild-type strain, the Nc-Spain7 isolate, using both cell populations. For the bovine trophoblast, no differences were observed in the growth of the defective parasites compared to the wild-type strain, neither in the proliferation kinetics nor in the competition assay. However, in naïve BoMØ, a significant decrease in the proliferation capacity of the mutant parasites was observed from 48 h pi onwards. Stimulation of BoMØ with IFN-γ showed a similar inhibition of tachyzoite growth in defective and wild-type strains in a dose-dependent manner. Finally, BoMØ infected with knockout parasites showed higher expression levels of TLR3, which is involved in pathogen recognition. These results suggest that NcGRA7 and NcROP40 may be involved in the manipulation of innate immune defense mechanisms against neosporosis and confirm the usefulness of the BoMØ model for the evaluation of N. caninum virulence mechanisms. However, the specific functions of these proteins remain unknown, opening the way for future research.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.doi10.1016/j.vetpar.2023.109973
dc.identifier.essn0304-4017
dc.identifier.issn1873-2550
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0304401723001048?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/88982
dc.journal.titleVeterinary Parasitology
dc.language.isoeng
dc.page.initial109973
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordNeospora caninum
dc.subject.keywordVirulence factor
dc.subject.keywordBovine trophoblast
dc.subject.keywordBovine macrophages
dc.subject.keywordNcGRA7 protein
dc.subject.keywordNcROP40 protein
dc.subject.ucmParasitología (Veterinaria)
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleCharacterization of Neospora caninum virulence factors NcGRA7 and NcROP40 in bovine target cells
dc.typejournal article
dc.volume.number320
dspace.entity.typePublication
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