Survey of Commercial Food Products for Detection of Walnut (Juglans regia) by Two ELISA Methods and Real Time PCR

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dc.contributor.authorMadrid García, Raquel
dc.contributor.authorGarcía García, Aina
dc.contributor.authorCabrera, Pablo
dc.contributor.authorGonzález, Isabel
dc.contributor.authorMartín, Rosario
dc.contributor.authorGarcía, Teresa
dc.date.accessioned2023-06-17T08:32:03Z
dc.date.available2023-06-17T08:32:03Z
dc.date.issued2021-02-17
dc.description.abstractLabeling of food allergens in accordance with legal regulations is important to protect the health of allergic consumers. The requirements for detecting allergens in foods involve adequate specificity and sensitivity to identify very small amounts of the target allergens in complex food matrices and processed foods. In this work, one hundred commercial samples were analyzed for walnut detection using three different methods: a sandwich enzyme-linked immunosorbent assay (ELISA) kit based on polyclonal antibodies, a direct ELISA using a recombinant multimeric scFv, and a real time PCR. The most sensitive method was real time PCR followed by sandwich ELISA kit and multimeric scFv ELISA. There was agreement between the three methods for walnut detection in commercial products, except for some heat-treated samples or those that contained pecan. The walnut ELISA kit was less affected by sample processing than was the multimeric scFv ELISA, but there was cross-reactivity with pecan, producing some false positives that must be confirmed by real time PCR. According to the results obtained, 7.0 to 12.6% of samples (depending on the analytical method) contained walnut but did not declare it, confirming there is a risk for allergic consumers. Moreover, there was one sample (3.7%) labelled as containing walnut but that tested negative for this tree nut. Genetic and immunoenzymatic techniques offer complementary approaches to develop a reliable verification for walnut allergen labeling.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMINECO
dc.description.sponsorshipComunidad de Madrid, Consejería de Educación
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77446
dc.identifier.doi10.3390/foods10020440
dc.identifier.issn2304-8158
dc.identifier.officialurlhttps://doi.org/10.3390/foods10020440
dc.identifier.relatedurlhttps://www.mdpi.com/2304-8158/10/2/440
dc.identifier.urihttps://hdl.handle.net/20.500.14352/7383
dc.issue.number2
dc.journal.titleFoods
dc.language.isoeng
dc.page.initial440
dc.publisherMPDI
dc.relation.projectIDAGL2017-84316-R
dc.relation.projectIDS2018/BAA-4574
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordELISA
dc.subject.keywordfood allergen labeling
dc.subject.keywordfood analysis
dc.subject.keywordpolyclonal antibody
dc.subject.keywordreal time PCR
dc.subject.keywordScFv recombinant antibody
dc.subject.keywordwalnut detection
dc.subject.ucmNutrición y cuidado de los animales
dc.subject.unesco3109.06 Nutrición
dc.titleSurvey of Commercial Food Products for Detection of Walnut (Juglans regia) by Two ELISA Methods and Real Time PCR
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublication92f11fce-d91b-47eb-8e14-bd4ce6c79cf6
relation.isAuthorOfPublication4c236a4b-c0dd-47b7-9bcd-4af6034724af
relation.isAuthorOfPublication.latestForDiscovery4c236a4b-c0dd-47b7-9bcd-4af6034724af
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