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Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci

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dc.contributor.authorBorrero Del Pino, Juan
dc.contributor.authorChen, Yuqing
dc.contributor.authorDunny, Gary M.
dc.contributor.authorKaznessis, Yiannis N.
dc.date.accessioned2025-01-27T18:12:51Z
dc.date.available2025-01-27T18:12:51Z
dc.date.issued2014-06-04
dc.description.abstractWe designed Lactococcus lactis to detect Enterococcus faecalis. Upon detection, L. lactis produce and secrete antienterococcal peptides. The peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of L. lactis. The enterococcal sex pheromone cCF10 serves as the signal for detection. Expression vectors derived from pCF10, a cCF10-responsive E. faecalis sex-pheromone conjugative plasmid, were engineered in L. lactis for the detection system. Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis. Sensitive detection and specific inhibition occur both in agar and liquid media. The engineered L. lactis also inhibited growth of multidrug-resistant E. faecium strains, when induced by cCF10. The presented vectors and strains can be components of a toolbox for the development of alternative antibiotic technologies targeting enterococci at the site of infection.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipNational Institutes of Health (EEUU)
dc.description.sponsorshipNational Science Foundation (EEUU)
dc.description.statuspub
dc.identifier.citationBorrero, J., Chen, Y., Dunny, G. M., & Kaznessis, Y. N. (2015). Modified lactic acid bacteria detect and inhibit multiresistant enterococci. ACS Synthetic Biology, 4(3), 299-306. https://doi.org/10.1021/SB500090B
dc.identifier.doi10.1021/sb500090b
dc.identifier.issn2161-5063
dc.identifier.issn2161-5063
dc.identifier.officialurlhttps://doi.org/10.1021/SB500090B
dc.identifier.urihttps://hdl.handle.net/20.500.14352/116433
dc.issue.number3
dc.journal.titleACS Synthetic Biology
dc.language.isoeng
dc.page.final306
dc.page.initial299
dc.publisherACS Publications
dc.relation.projectIDGM08686
dc.relation.projectIDGM04953
dc.relation.projectIDCBET-0644792
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu579
dc.subject.keywordLactic acid bacteria
dc.subject.keywordLactococcus lactis
dc.subject.keywordEnterococcus faecalis
dc.subject.keywordDrug delivery vector
dc.subject.keywordRecombinant plasmids
dc.subject.ucmMicrobiología (Biología)
dc.subject.unesco3302.03 Microbiología Industrial
dc.titleModified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number4
dspace.entity.typePublication
relation.isAuthorOfPublicationadc58430-a38c-4344-992e-4672985881b7
relation.isAuthorOfPublication.latestForDiscoveryadc58430-a38c-4344-992e-4672985881b7

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