Simultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms

Simple item page
dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorLópez-Hernández, Eva
dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorGranados, Rosario
dc.contributor.authorSánchez-Puelles, Jose Maria
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-29T11:03:14Z
dc.date.available2024-01-29T11:03:14Z
dc.date.issued2015
dc.descriptionLos resultados derivados de esta investigación fueron empleados por la Oficina de Transferencia de Resultados de Investigación de la UCM para la elaboración de una noticia con título: 'Un biosensor magnético detecta el cáncer de mama más agresivo'. Además, este artículo fue resaltado en numerosos sitios web, poniendo de manifiesto su indiscutible relevancia. Algunos de los sitios en los que la noticia fue publicaba se incluyen a continuación: https://www.agenciasinc.es/Noticias/Un-biosensor-magnetico-detecta-el-cancer-de-mama-mas-agresivo https://revistalvr.es/un-biosensor-magnetico-detecta-el-cancer-de-mama-mas-agresivo/ https://www.biotechmagazine.es/noticias-biotech/biosensor-magnetico-frente-al-cancer-de-mama/ https://www.infosalus.com/asistencia/noticia-biosensor-detectar-cancer-mama-mas-agresivo-made-in-spain-20150422183529.html https://www.rtve.es/noticias/20150422/biosensor-magnetico-detecta-cancer-mama-mas-agresivo-solo-15-minutos/1134380.shtml https://www.bolsamania.com/noticias/sanidad/disenan-un-biosensor-magnetico-capaz-de-detectar-precozmente-el-cancer-de-mama-mas-agresivo--699233.html http://biotech-spain.com/en/articles/un-biosensor-magn-tico-detecta-el-c-ncer-de-mama-m-s-agresivo/
dc.description.abstractA novel magnetobiosensing approach for the rapid and simultaneous detection of two breast cancer-related miRs (miR-21 and miR-205) is reported. It involves the use of antimiR-21 and antimiR-205 specific probes, chitin-modified magnetic beads (Chitin-MBs), the p19 viral protein as capture bioreceptor and amperometric detection with the H2O2/hydroquinone (HQ) system at dual screen-printed carbon electrodes (SPdCEs). The use of SPdCEs allows the simultaneous independent amperometric readout for each target miR to be measured. The magnetosensor exhibited sensitive and selective detection with dynamic ranges from 2.0 to 10.0nM and detection limits of 0.6nM (6fmol) for both target miRs without any amplification step in less than 2h. The usefulness of the approach was evaluated by detecting the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer cell lines and human tissues.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipMinisterio de Educación (España)
dc.description.statuspub
dc.identifier.citationTorrente-Rodríguez RM, Campuzano S, López-Hernández E, Montiel VR, Barderas R, Granados R, Sánchez-Puelles JM, Pingarrón JM. Simultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms. Biosens Bioelectron. 2015 Apr 15;66:385-91. doi: 10.1016/j.bios.2014.11.047. Epub 2014 Nov 26. PMID: 25481114.
dc.identifier.doi10.1016/j.bios.2014.11.047
dc.identifier.issn0956-5663
dc.identifier.officialurlhttp://doi.org/10.1016/j.bios.2014.11.047
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95881
dc.journal.titleBiosensors & Bioelectronics
dc.language.isoeng
dc.page.final391
dc.page.initial385
dc.publisherElsevier
dc.relation.projectIDCTQ2012-34238
dc.relation.projectIDAES12/PI12/01628
dc.relation.projectIDS2013/MT- 3029
dc.relation.projectIDCM S2011/BMED-2353
dc.relation.projectIDFP7-HEALTH- 2012-INNOVATION-1
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordMiRNA-21
dc.subject.keywordMiRNA-205
dc.subject.keywordP19
dc.subject.keywordSimultaneous detection
dc.subject.keywordMagnetic beads
dc.subject.keywordSPdCEs
dc.subject.keywordCancer samples
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleSimultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number66
dspace.entity.typePublication
relation.isAuthorOfPublicationf4569700-ddad-4754-a155-145a8107c215
relation.isAuthorOfPublication6945fb1e-9c48-459d-b994-e64328c3e79b
relation.isAuthorOfPublicationf7757d94-e7fd-4416-9a9f-4c2539dc2fd4
relation.isAuthorOfPublication21f5c8af-61fb-4d35-8e88-c01ee15a3bba
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscoveryf4569700-ddad-4754-a155-145a8107c215
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Simultaneous_detection_of_two_breast_cancer-related_miRNAs.pdf
Size:
733.29 KB
Format:
Adobe Portable Document Format
Download
Collections
Artículos