Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

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dc.contributor.authorSánchez Tirado, E.
dc.contributor.authorMartínez García, G.
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-17T22:41:57Z
dc.date.available2023-06-17T22:41:57Z
dc.date.issued2017
dc.description.abstractThe first amperometric immunosensor for the quantification of TGF-β1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-β1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-β1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-β1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/55219
dc.identifier.doi10.1016/j.bios.2016.05.093
dc.identifier.issnISSN: 0956-5663, ESSN: 1873-4235
dc.identifier.officialurlhttps://www.ncbi.nlm.nih.gov/pubmed/27297187
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18770
dc.journal.titleBiosensors and Bioelectronics
dc.language.isoeng
dc.page.final14
dc.page.initial9
dc.publisherElsevier
dc.relation.projectIDCTQ2015–70023-R
dc.relation.projectIDNANOAVANSENS (S2013/MT-3029)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu543
dc.subject.keywordElectrochemical immunosensor
dc.subject.keywordSignal amplification
dc.subject.keywordTransforming growth factor-β1 (TGF-β1)
dc.subject.keywordUrine
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine
dc.typejournal article
dc.volume.number88
dspace.entity.typePublication
relation.isAuthorOfPublication8a730734-cdf5-4b59-b017-12e2320c872e
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery8a730734-cdf5-4b59-b017-12e2320c872e
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