Properties of virulence emergence of Leishmania infantum isolates from Phlebotomus perniciosus collected during the human leishmaniosis outbreak in Madrid, Spain. Hepatic histopathology and immunological parameters as virulence markers in the mouse model

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dc.contributor.authorMaribel Jiménez
dc.contributor.authorMas Zubiri, Alicia
dc.contributor.authorMartínez Rodrigo, Abel
dc.contributor.authorOrden Gutiérrez, José Antonio
dc.contributor.authorCarrión Herrero, Francisco Javier
dc.contributor.authorDomínguez Bernal, Gustavo Ramón
dc.contributor.authorJiménez Martínez, María De Los Ángeles
dc.date.accessioned2024-01-11T14:18:20Z
dc.date.available2024-01-11T14:18:20Z
dc.date.issued2020-08
dc.description.abstractRecent anthropic activity related to the construction of the Bosquesur Green Park in a large urban setting in Madrid (Spain) has resulted in the largest reported community outbreak of human leishmaniosis in Europe. Previous phylogenetic and molecular-typ-ing studies of parasite isolates have implicated the Leishmania infantum ITS-Lombardi genotype in this outbreak. In an unusual scenario, visceral leishmaniosis (VL) is affecting a significant number of individuals, suggesting that an increase in parasite virulence has occurred. In this work, using an in vivo BALB/c model of VL, we aimed to investigate the properties of emergent virulence of the L. infantum POL2FL7 and BOS1FL1 isolates obtained from Phlebotomus perniciosus collected in the outbreak area and compare them with those of the well-characterized strain BCN150 MON-1 isolated from a dog. The P. perniciosus specimens were collected during an entomological survey conducted in the transmission season of 2012. We observed a range of virulence phenotypes from moderately to highly aggressive after 5 weeks of infection. IV challenge of mice with outbreak isolates from sand flies induced higher splenic and liver parasite burdens, higher serological titres of specific anti-Leishmania antibodies and impaired capacities to control infection, as revealed by the arginine metabolism and low ratios of Th1/Th2 cy-tokine profiles analysed, compared with the corresponding measures evaluated in mice infected with the BCN150 strain. The BOS1FL1 isolate showed the highest degree of virulence among the isolates, superior to that of POL2FL7, as evidenced by the analysed biomarkers and the histopathological severity of liver lesions. These results provide in-sight into how L. infantum isolates from sand flies collected in the outbreak area have been able to affect not only immunosuppressed patients but also middle-aged people with normal immunocompetence in the largest human VL outbreak in Europe.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUCM-SANTANDER
dc.description.sponsorshipCOMUNIDAD DE MADRID
dc.description.statuspub
dc.identifier.citationMolina-Rueda F, Fernández-González P, Cuesta-Gómez A, Koutsou A, Carratalá-Tejada M, Miangolarra JC. Test-Retest Reliability of a Conventional Gait Model for Registering Joint Angles during Initial Contact and Toe-Off in Healthy Subjects. Int J Environ Res Public Health. 2021 Feb 15;3(18):1343-52
dc.identifier.doi10.1111/tbed.13733
dc.identifier.essn1865-1682
dc.identifier.issn1865-1674
dc.identifier.officialurlhttps://onlinelibrary.wiley.com/journal/18651682
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92564
dc.journal.titleTransboundary and Emerging Diseases
dc.language.isoeng
dc.page.final714
dc.page.initial704
dc.publisherWiley
dc.relation.projectIDPR75/18-21558 (UCM-SANTANDER)
dc.relation.projectIDPLATESA2-CM (S2018/BAA-4370) (COMUNIDAD DE MADRID)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsembargoed access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu616.993.161
dc.subject.cdu616.993
dc.subject.keywordBosquesur
dc.subject.keywordLeishmania infantum
dc.subject.keywordLeishmaniosis
dc.subject.keywordOutbreak
dc.subject.keywordPhlebotomus perniciosus
dc.subject.keywordVirulence
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.titleProperties of virulence emergence of Leishmania infantum isolates from Phlebotomus perniciosus collected during the human leishmaniosis outbreak in Madrid, Spain. Hepatic histopathology and immunological parameters as virulence markers in the mouse model
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number68
dspace.entity.typePublication
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