Characterization of Ghrelin O-Acyltransferase (GOAT) in goldfish (Carassius auratus)

Simple item page
dc.contributor.authorBlanco Imperiali, Ayelén M.
dc.contributor.authorGómez Boronat, Miguel
dc.contributor.authorAlonso Gómez, Ángel Luis
dc.contributor.authorYufa, Roman
dc.contributor.authorUnniappan, Suraj
dc.contributor.authorDelgado Saavedra, María Jesús
dc.contributor.authorValenciano González, Ana Isabel
dc.date.accessioned2023-06-17T22:04:40Z
dc.date.available2023-06-17T22:04:40Z
dc.date.issued2017-02-08
dc.description.abstractGhrelin is the only known hormone posttranslationally modified with an acylation. This modification is crucial for most of ghrelin’s physiological effects and is catalyzed by the polytopic enzyme ghrelin O-acyltransferase (GOAT). The aim of this study was to characterize GOAT in a teleost model, goldfish (Carassius auratus). First, the full-length cDNA sequence was obtained by RT-PCR and rapid amplification of cDNA ends methods. Two highly homologous cDNAs of 1491 and 1413 bp, respectively, named goat-V1 and goat-V2 were identified. Deduced protein sequences (393 and 367 amino acids, respectively) are predicted to present 11 and 9 transmembrane regions, respectively, and both contain two conserved key residues proposed to be involved in catalysis: asparagine 273 and histidine 304. RT-qPCR revealed that both forms of goat mRNAs show a similar widespread tissue distribution, with the highest expression in the gastrointestinal tract and gonads and less but considerable expression in brain, pituitary, liver and adipose tissue. Immunostaining of intestinal sections showed the presence of GOAT immunoreactive cells in the intestinal mucosa, some of which colocalize with ghrelin. Using an in vitro approach, we observed that acylated ghrelin downregulates GOAT gene and protein levels in cultured intestine in a time-dependent manner. Finally, we found a rhythmic oscillation of goat mRNA expression in the hypothalamus, pituitary and intestinal bulb of goldfish fed at midday, but not at midnight. Together, these findings report novel data characterizing GOAT, and offer new information about the ghrelinergic system in fish.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canada
dc.description.sponsorshipSaskatchewan Health Research Foundation (SHRF)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44376
dc.identifier.doi10.1371/journal.pone.0171874
dc.identifier.issn1932-6203
dc.identifier.officialurlhttp://journals.plos.org/plosone/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18024
dc.issue.number2
dc.journal.titlePlos ONE
dc.language.isoeng
dc.page.final28
dc.page.initial1
dc.publisherPublic Library of Science
dc.relation.projectIDAGL2016-74857 C3-2-R
dc.relation.projectIDDiscovery Grant (341292-2012-RGPIN) and Discovery Accelerator Supplement (429436-2012-RGPAS)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu591.1
dc.subject.cdu597.2/.5
dc.subject.keywordCarassius auratus
dc.subject.keywordGhrelin O-Acyltransferase (GOAT)
dc.subject.ucmBiología
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmPeces
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2401.13 Fisiología Animal
dc.titleCharacterization of Ghrelin O-Acyltransferase (GOAT) in goldfish (Carassius auratus)
dc.typejournal article
dc.volume.number12
dspace.entity.typePublication
relation.isAuthorOfPublication44ac4b2f-08dc-44a8-8d08-cea0a94715f7
relation.isAuthorOfPublication71c5047d-4eb1-48ac-86d4-21c7b2d335a7
relation.isAuthorOfPublication48071251-a236-4607-ab33-5332c1472628
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication.latestForDiscovery44ac4b2f-08dc-44a8-8d08-cea0a94715f7
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Valenciano. 2017. Characterization of Ghrelin O Acyltransferase (GOAT) in goldfish (Carassius auratus).pdf
Size:
5.38 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos