Evaluation of polyesteramide (PEA) and polyester (PLGA) microspheres as intravitreal drug delivery systems in albino rats

Simple item page
dc.contributor.authorPeters, Tobias
dc.contributor.authorKim, Seong-Woo
dc.contributor.authorCastro, Vinicius
dc.contributor.authorStingl, Krunoslav
dc.contributor.authorStrasser, Torsten
dc.contributor.authorBolz, Sylvia
dc.contributor.authorSchraermeyer, Ulrich
dc.contributor.authorMihov, George
dc.contributor.authorZong, MengMeng
dc.contributor.authorAndrés Guerrero, Vanesa
dc.contributor.authorHerrero Vanrell, María Del Rocío
dc.contributor.authorDias, Aylvin
dc.contributor.authorCameron, Neil
dc.contributor.authorZrenner, Eberhart
dc.date.accessioned2023-06-17T22:40:16Z
dc.date.available2023-06-17T22:40:16Z
dc.date.issued2017-04
dc.description.abstractPurpose: To study the suitability of injectable microspheres based on poly(ester amide) (PEA) or poly lactic-co-glycolic acid (PLGA) as potential vehicles for intravitreal drug delivery in rat eyes. Dexamethasone loaded PEA microspheres (PEA+DEX) were also evaluated. Methods: Forty male Sprague Dawley rats were divided into four groups that received different intravitreally injected microspheres: PEA group (n=12); PLGA group (n=12); PEA+DEX group (n=8); and control group (no injection, n=8). Electroretinography (ERG), fundus autofluorescence (FAF), and spectral domain optical coherence tomography (sdOCT) were performed at baseline, weeks 1 and 2, and months 1, 2, and 3 after intravitreal injection. Eyes were histologically examined using light microscopy and transmission electron microscopy at the end of the in vivo study. Results: There were no statistically significant changes in ERG among the groups. Abnormal FAF pattern and abnormal deposits in OCT were observed after injection but almost completely disappeared between week 2 and month 3 in all injected groups. GFAP staining showed that Müller glia cell activation was most pronounced in PLGA-injected eyes. Increased cell death was not observed by TUNEL staining at month 1. In electron microscopy at month 3, the remnants of microparticles were found in the retinal cells of all injected groups, and loss of plasma membrane was seen in the PLGA group. Conclusions: Although morphological changes such as mild glial activation and material remnants were observed histologically 1 month and 3 months after injection in all injected groups, minor cell damage was noted only in the PLGA group at 3 months after injection. No evidence of functional abnormality relative to untreated eyes could be detected by ERG 3 months after injection in all groups. Changes observed in in vivo imaging such as OCT and FAF disappeared after 3 months in almost all cases.en
dc.description.departmentDepto. de Farmacia Galénica y Tecnología Alimentaria
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea
dc.description.sponsorshipTistou and Charlotte Kerstan Foundation
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/51772
dc.identifier.citationPeters, T. Kim, S., Castro, V. et al. «Evaluation of Polyesteramide (PEA) and Polyester (PLGA) Microspheres as Intravitreal Drug Delivery Systems in Albino Rats». Biomaterials, vol. 124, abril de 2017, pp. 157-68. DOI.org (Crossref), https://doi.org/10.1016/j.biomaterials.2017.02.006.
dc.identifier.doi10.1016/j.biomaterials.2017.02.006
dc.identifier.issn0142-9612
dc.identifier.officialurlhttp://doi.org/10.1016/j.biomaterials.2017.02.006
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18744
dc.journal.titleBiomaterials
dc.language.isoeng
dc.page.final168
dc.page.initial157
dc.publisherElsevier
dc.relation.projectIDPeptide-based Nanoparticles as Ocular Drug Delivery Vehicles (PANOPTES)
dc.relation.projectIDNMP4-SL- 2010-246180
dc.rights.accessRightsopen access
dc.subject.cdu615.4
dc.subject.keywordPolyesteramide based on α-amino acids
dc.subject.keywordAliphatic dicarboxylic acids and aliphatic α-ω diols (PEA)
dc.subject.keywordPoly lactic-co-glycolic acid (PLGA)
dc.subject.keywordInvivo electroretinography
dc.subject.keywordInvivo optical coherence tomography
dc.subject.keywordFundus auto-fluorescence
dc.subject.keywordImmunohistochemistry
dc.subject.keywordTUNEL stain
dc.subject.keywordTransmission electron microscopy
dc.subject.ucmFarmacia
dc.subject.ucmFarmacología (Farmacia)
dc.subject.ucmTecnología farmaceútica
dc.subject.unesco3209 Farmacología
dc.titleEvaluation of polyesteramide (PEA) and polyester (PLGA) microspheres as intravitreal drug delivery systems in albino ratsen
dc.typejournal article
dc.volume.number124
dspace.entity.typePublication
relation.isAuthorOfPublication14096cb3-8372-40d2-8fe1-79d0c4c1ddd7
relation.isAuthorOfPublicatione19672b5-d6f7-400a-b591-b903bc396955
relation.isAuthorOfPublication.latestForDiscoverye19672b5-d6f7-400a-b591-b903bc396955
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
evaluation of polyesteramide (PEA) and polyester (PLGA) microspheres as intravitreal drug delivery systems in albino rats.pdf
Size:
6.93 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos